四川大学学报（自然科学版）2026，Vol.63Issue(2)：308-315,8.DOI:10.19907/j.0490-6756.250340
线粒体复合物Ⅰ亚基NDUFS4在东亚三角涡虫再生中的功能解析
The functional role of mitochondrial complex Ⅰ subunit NDUFS4 in planarian(Dugesia sp.)regeneration
摘要
Abstract
This study aimed to elucidate the role of NADH dehydrogenase(ubiquinone)iron-sulfur protein 4(NDUFS4),a core structural subunit of mitochondrial complex Ⅰ(CⅠ),in maintenance of adult stem cell function and neural regeneration in planarians.The ndufs4 gene was cloned from Dugesia sp.,and sequence together with structural prediction analyses revealed that the encoded protein is highly conserved in canonical domains and key amino acid residues.To assess its function during regeneration,RNA interference was used to knockdown the ndufs4 expression.Blastema growth rates in regenerating planarians were quantified,and in situ hybridization and immunohistochemistry were performed to assess adult stem cell(Neoblast)prolifera-tion and differentiation,as well as neural regeneration.The results showed that ndufs4 knockdown signifi-cantly reduced regeneration speed and severely impaired neural regeneration.Concurrently,the expression levels of stem cell marker genes were markedly decreased,and stem cell differentiation was disrupted.To-gether,these findings indicate that NDUFS4 is an essential for maintaining the adult stem cell population and promoting neural regeneration in planarians,highlighting its critical regulatory role in biological regeneration.关键词
东亚三角涡虫/NADH脱氢酶铁硫蛋白4/再生/干细胞增殖与分化Key words
Dugesia sp./NADH dehydrogenase(Ubiquinone)Fe S protein 4/regeneration/stem cell pro-liferation and differentiation分类
生物科学引用本文复制引用
于萍,高丽丽,庞秋香,李奥,王金蕾,孟宪鹏,张颖,张子怡,孙雪平,魏文硕,张永乐,马秀雪..线粒体复合物Ⅰ亚基NDUFS4在东亚三角涡虫再生中的功能解析[J].四川大学学报（自然科学版）,2026,63(2):308-315,8.基金项目
国家自然科学基金面上项目(32470518) （32470518）
山东省自然科学基金面上项目(ZR2022MC022,ZR2024MC154) （ZR2022MC022,ZR2024MC154）
