热带作物学报2026，Vol.47Issue(2)：364-371,8.DOI:10.3969/j.issn.1000-2561.2026.02.008
Flg22重组蛋白的原核诱导及与flg22短肽的功能比较
Prokaryotic Induction of Recombinant Flg22 Protein and Functional Comparative Analysis with flg22 Peptide
摘要
Abstract
Flg22 is an artificially synthesized 22-amino-acid peptide derived from the conserved N-terminal region of bacterial flagellin,playing a crucial role in regulating plant immune responses.Due to the high cost of artificially syn-thesized flg22 short peptides and limitations for large-scale application,this study aimed to determine the suitable con-ditions for prokaryotic induction expression of the flg22 protein and to verify its function,thereby determining whether it could replace the commercially used flg22 short peptides.In this study,the pET32a-flg22 prokaryotic expression vector was synthesized,and we then compared protein expression efficiency under induction at 28℃and 37℃.Addi-tionally,we detected the dynamic burst of reactive oxygen species(ROS)in cassava treated with flg22 peptides and flg22 proteins at different concentrations to validate the functions.The results indicated that 28℃was the optimal in-duction temperature for inducing flg22 protein expression.ROS measurements showed that within a specific concentra-tion range,the peak intensity of ROS increased with the increase in flg22 protein concentration.Furthermore,after spraying cassava leaves with 60 μg/mL and 600 μg/mL of flg22 recombinant proteins,as well as 2.27 μg/mL of flg22 peptides,for three days,we inoculated plants with the pathogen Xam(Xanthomonas axonopodis pv.manihotis,Xam).Compared to treatment with sterile water,both flg22 protein and flg22 peptide treatments showed significant antibacte-rial effects against Xam in cassava plants,with the 60 μg/mL and 600 μg/mL flg22 protein treatments showing notably stronger antibacterial activity than the flg22 peptide.The results preliminarily identified the prokaryotic expression conditions of flg22 recombinant protein and verified that its function in inducing plant defense responses is consistent with that of the peptide,providing a theoretical basis for substituting commercial peptides with flg22 recombinant protein.关键词
原核诱导表达/flg22/短肽/病原菌/ROSKey words
prokaryotic induced expression/flg22/peptide/pathogen/reactive oxygen species分类
农业科技引用本文复制引用
王萌媛,程科,杨澜,赵惠萍..Flg22重组蛋白的原核诱导及与flg22短肽的功能比较[J].热带作物学报,2026,47(2):364-371,8.基金项目
海南省教育厅项目(No.Hnky2024-10) （No.Hnky2024-10）
海南大学南繁学院创新创业训练项目(No.NFJD2024-15). （No.NFJD2024-15）
