世界中西医结合杂志2026，Vol.21Issue(1)：53-59,7.DOI:10.13935/j.cnki.sjzx.260110
基于SCF/c-Kit通路探讨加味安肠汤联合阿洛司琼对腹泻型肠易激综合征大鼠的作用机制
Mechanisms of Modified Anchang Decoction Combined with Alosetron on Diarrhea-Predominant Irritable Bowel Syndrome in Rats Based on SCF/c-Kit Pathway
摘要
Abstract
Objective To investigate the mechanisms of action of modified Anchang Decoction combined with alosetron in rats with diarrhea-predominant irritable bowel syndrome(IBS-D)based on the stem cell factor(SCF)/cellular tyrosine kinase receptor(c-Kit)signaling pathway.Methods A total of 100 SPF Wistar rats of the same age were used,of which 80 rats were randomly selected for modeling to establish an IBS-D rat model.After successful modeling,the rats were randomly divided,using a random number table,into a model group(intragastric administration of 10 ml/kg normal saline),an alosetron group(intragastric administration of 1.008 mg/kg),a modified Anchang Decoction group(intragastric administration of 18.9 g/kg modified Anchang Decoction),and an integrated traditional Chinese and Western medicine group(intragastric administration of 1.008 mg/kg alosetron combined with 18.9 g/kg modified Anchang Decoction),with 20 rats in each group.The remaining 20 rats served as the blank control group.Eight hours after the final treatment,rats in each group were anesthetized with 10%chloral hydrate,sacrificed,and 10 ml of abdominal aortic blood and colon tissues were collected.The general conditions of the rats,as well as levels of 5-Hydroxytryptamine(5-HT)and SCF,were observed.Immunofluorescence was used to determine the levels of c-Kit-positive interstitial cells of Cajal(ICC),and Western blot analysis was used to measure SCF and c-Kit protein expression in each group.Results Compared with the blank group,the model group showed significantly reduced body weight,SCF levels,ICC fluorescence intensity,and SCF and c-Kit protein expression,while 5-HT levels were significantly increased(P<0.05),indicating successful model establishment.After modeling,SCF levels,ICC fluorescence intensity,and SCF and c-Kit protein expression showed abnormal increases,while 5-HT levels showed abnormal decreases.After 1 and 2 weeks of treatment,body weight in the alosetron group,modified Anchang Decoction group,and integrated traditional Chinese and Western medicine group was significantly increased compared with the model group(P<0.05).Compared with the alosetron group and modified Anchang Decoction group,body weight in the integrated traditional Chinese and Western medicine group was significantly increased(P<0.05).Compared with the model group,5-HT levels were decreased,while SCF levels,ICC fluorescence intensity,and SCF and c-Kit protein expression were increased in the alosetron group,modified Anchang Decoction group,and integrated treatment group(P<0.05).Compared with the alosetron group and modified Anchang Decoction group,the integrated traditional Chinese and Western medicine group showed significantly lower 5-HT levels and higher SCF levels,ICC fluorescence intensity,and SCF and c-Kit protein expression(P<0.05).Conclusion Modified Anchang Decoction combined with alosetron exerts therapeutic effects on IBS-D through the SCF/c-Kit signaling pathway.关键词
SCF/c-Kit通路/加味安肠汤/阿洛司琼/腹泻型肠易激综合征Key words
SCF/c-Kit pathway/Modified Anchang Decoction/alosetron/diarrhea-predominant irritable bowel syndrome分类
医药卫生引用本文复制引用
袁小晶,王凡..基于SCF/c-Kit通路探讨加味安肠汤联合阿洛司琼对腹泻型肠易激综合征大鼠的作用机制[J].世界中西医结合杂志,2026,21(1):53-59,7.基金项目
第六批全国老中医药专家学术经验继承工作项目(国中医药人教发[2018]29号) （国中医药人教发[2018]29号）
