时珍国医国药2026，Vol.37Issue(4)：634-640,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-0405
温阳生肌膏通过p38MAPK/NF-κB信号通路减轻糖尿病创面炎症反应的研究
Wenyang Shengji Ointment(温阳生肌膏)alleviates inflammation in diabetic wounds via the p38 MAPK/NF-κB signaling pathway
摘要
Abstract
Objective To explore the mechanism underlying the regulation of the p38 MAPK/NF-κB signaling pathway by Wenyang Shengji Ointment(温阳生肌膏,WYSGO)in ameliorating inflammation in high glucose-induced macrophage-fibroblast cocultures.Methods Models of high glucose-treated RAW264.7,NIH3T3,and their co-culture system were established.The cells were divided into normal,model,and WYSGO groups.Cell proliferation,migration,macrophage polarization,TNF-α content,and related protein expression were assessed by CCK-8 assay,scratch wound healing assay,flow cytometry,ELISA,and Western blotting(WB),respec-tively.Results Compared with the model group,WYSGO significantly promoted cell proliferation(P<0.01 or P<0.001),inhibited M1 polarization of RAW264.7 cells(P<0.001),and promoted their M2 polarization(P<0.001).In addition,WYSGO restored the migra-tion ability of NIH3T3 and co-cultured cells(P<0.01),downregulated the expression of p38 MAPK and NF-κB p65 proteins in NIH3T3 cells(P<0.05),and significantly reduced the levels of p38 MAPK,NF-κB p65,and phosphorylated NF-κB p65(p-NF-κB p65)proteins in RAW264.7 and co-cultured cells(P<0.05,P<0.01,or P<0.001),as well as the level of the inflammatory factor TNF-α(P<0.001).Conclusion Fibroblast dysfunction is alleviated by WYSGO through the suppression of the p38 MAPK/NF-κB path-way activation,which subsequently reverses macrophage M1 polarization,reduces TNF-α release,and blocks the transmission of inflammatory signals in macrophage-fibroblast interactions.关键词
温阳生肌膏/p38 MAPK/NF-κB/巨噬细胞/成纤维细胞/炎症反应Key words
Wenyang Shengji Ointment(温阳生肌膏)/p38 MAPK/NF-κB/macrophages/fibroblasts/inflammatory response分类
医药卫生引用本文复制引用
宫海燕,王君,丁雅容,黄新宇,师思,蒋雨洋,奉水华,陈丽..温阳生肌膏通过p38MAPK/NF-κB信号通路减轻糖尿病创面炎症反应的研究[J].时珍国医国药,2026,37(4):634-640,7.基金项目
海南省重点研发项目(ZDYF2023SHFZ123) （ZDYF2023SHFZ123）
湖南省教育厅重点项目(24A0273) （24A0273）
湖南省中医药科研课题(C2023038) （C2023038）
湖南中医药大学研究生创新课题(2024CX195) （2024CX195）
湖南中医药大学省级学科建设项目(中西医结合) （中西医结合）
血管生物学与转化医学湖南省重点实验室项目(2023TP1018) （2023TP1018）
