上海中医药杂志2026，Vol.60Issue(3)：71-80,87,11.DOI:10.16305/j.1007-1334.2026.z20250711002
层次分析法‑熵权法结合Box‑Behnken响应面法优化加味桔梗汤水提醇沉工艺
Optimization of water extraction and ethanol precipitation process for modified Jiegeng Decoction by combination of AHP‑entropy weight method and Box‑Behnken design‑response surface method
摘要
Abstract
Objective To establish a validated ultra-performance liquid chromatography(UPLC)method for the simultaneous quantification of multiple components in modified Jiegeng Decoction and to optimize its water extraction and ethanol precipitation processes using the Box-Behnken design-response surface method(BBD-RSM).Methods Platycodin D was determined by UPLC coupled with evaporative light scattering detection(UPLC-ELSD),while gallic acid,chlorogenic acid,corilagin,hesperidin,and rosmarinic acid were quantified via UPLC with ultraviolet detection(UPLC-UV).Weight coefficients for each evaluation index were calculated using the analytic hierarchy process(AHP)-entropy weight method.Based on the results of single-factor experiments,BBD-RSM was employed to investigate the effects of soaking time and decoction time on the water extraction process,as well as the impacts of ethanol concentration,precipitation time,and extract density on the ethanol precipitation process.Results A UPLC method for the simultaneous determination of multiple components in modified Jiegeng Decoction was successfully established and validated.The optimized water extraction conditions were confirmed as follows:no soaking,12-fold volume of water relative to the raw material,and two rounds of decoction(65 minutes per round).For ethanol precipitation,the optimal parameters were determined to be 80%(V/V)ethanol concentration,48 hours of precipitation,and an extract density of 1.65 g·mL-1.Conclusions The established UPLC method exhibits high accuracy and reliability,and the optimized extraction-precipitation parameters demonstrate good stability.This study provides a solid technical foundation for subsequent pharmacological research and large-scale industrial production of Modified Jiegeng Decoction.关键词
急性咽炎/加味桔梗汤/水提醇沉工艺优化/含量测定/中药研究Key words
acute pharyngitis/modified Jiegeng Decoction/optimization of water extraction and ethanol precipitation processes/content determination/Chinese materia medica research引用本文复制引用
王君怡,郑敏,何国华,程雪梅,王长虹..层次分析法‑熵权法结合Box‑Behnken响应面法优化加味桔梗汤水提醇沉工艺[J].上海中医药杂志,2026,60(3):71-80,87,11.基金项目
新疆维吾尔自治区科技厅新疆丝绸之路经济带创新驱动发展试验区、乌昌石国家自主创新示范区科技发展计划(新疆特色药材资源可持续利用与品质评价关键技术研究)项目(2024LQ03007) （新疆特色药材资源可持续利用与品质评价关键技术研究）
嘉兴复道致德医疗科技有限公司委托项目(2023310031004645) （2023310031004645）
