时珍国医国药2026，Vol.37Issue(4)：649-655,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-0407
葛根提取物通过基质细胞衍生因子-1调控正畸大鼠牙周膜干细胞增殖和成骨分化的研究
Pueraria lobata extract promotes proliferation and osteogenesis of periodontal ligament stem cells under orthodontic static pressure via SDF-1 in rats
摘要
Abstract
Objective To investigate whether Pueraria lobata extract(PLE)affects the proliferation and osteogenic differentiation of periodontal ligament stem cells(PDLSCs)under orthodontic static pressure by modulating stromal cell-derived factor-1(SDF-1)expres-sion.Methods PDLSCs were isolated from the periodontal ligament of the first molars of SD rats.Third-passage PDLSCs were subjected to orthodontic static pressure(OSP)and divided into the following groups:control(NC,no treatment),OSP,PLE-L(OSP+25 µg/mL PLE for 24 h),PLE-H(OSP+50 µg/mL PLE for 24 h),AMD3100(OSP+20 µmol/L SDF-1 inhibitor AMD3100 for 24 h),and PLE-H+AMD3100(OSP+50 µg/mL PLE and 20 µmol/L AMD3100 for 24 h).Cell proliferation was assessed using the CCK-8 assay.Alka-line phosphatase(ALP)activity was measured by colorimetry,and mineralization was evaluated by Alizarin red staining.The protein levels of proliferating cell nuclear antigen(PCNA),osteocalcin(OCN),osteopontin(OPN),RUNX family transcription factor 2(Runx2),and SDF-1 were determined by Western blot(WB)analysis.Results Compared with the NC group,the OSP group showed significant increases in OD450 values,ALP activity,mineralized nodule formation,and the protein expression levels of PCNA,OCN,OPN,Runx2,and SDF-1(all P<0.05).Compared with the OSP group,the PLE-L/H groups exhibited further enhancements in these same parameters(all P<0.05).In contrast,the AMD3100 group showed decreases in all measured outcomes compared to the OSP group(P<0.001).Furthermore,AMD3100 treatment attenuated the promoting effects of high-dose PLE on PDLSC proliferation and osteogenic differentiation.Conclusion PLE may promote the proliferation and osteogenic differentiation of PDLSCs in rats stimulated by orthodontic static pressure through up-regulation of SDF-1.关键词
葛根提取物/配体基质细胞衍生因子-1/牙周膜干细胞/成骨分化/增殖/正畸Key words
Pueraria lobata extract/Stromal cell-derived factor-1/Periodontal ligament stem cells/Osteogenic differentia-tion/Proliferation/Orthodontic static pressure分类
医药卫生引用本文复制引用
李慧,晏全红,金松,徐萍..葛根提取物通过基质细胞衍生因子-1调控正畸大鼠牙周膜干细胞增殖和成骨分化的研究[J].时珍国医国药,2026,37(4):649-655,7.基金项目
武汉市卫生和计划生育委员会科研项目(WX18C06) （WX18C06）
