时珍国医国药2026，Vol.37Issue(3)：417-423,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-0303
新风胶囊调节PI3K/AKT信号通路减轻骨关节炎大鼠炎症及骨代谢紊乱的机制研究
Mechanism of Xinfeng Capsule(新风胶囊)in regulating the PI3K/AKT signaling pathway to alleviate inflammation and bone metabolism disorder in osteoarthritis rats
摘要
Abstract
Objective To investigate the effects of Xinfeng Capsule(新风胶囊,XFC)on inflammation and bone metabolism in osteoar-thritis(OA)rats via the PI3K/AKT signaling pathway.Methods Sixty-four SD rats were randomly divided into eight groups:the Control group,Model group,low/medium/high-dose XFC(XFC-L,XFC-M,XFC-H)groups,celecoxib group,AKT activator SC79 group,and XFC-H+SC79 group.The OA Model was induced in all groups except the Control group by intra-articular injection of collagenase.Cartilage histology was analyzed using Safranin O/Fast Green and Alcian blue staining.Serum levels of TGF-β1,TNF-α,IL-1β,IL-6,TRACP-5b,IGF-1,OPG,and BALP were measured by ELISA.The protein expression levels of PI3K,p-PI3K,AKT,and p-AKT in cartilage were detected by Western blot(WB)analysis.Results Compared with the Control group,the Model group presented with a rough cartilaginous surface,reduced chondrocytes and extracellular matrix,and a destroyed tidemark.The expression levels of IL-1β,IL-6,TNF-α,TGF-β1,TRACP-5b,p-PI3K,and p-AKT were significantly elevated(P<0.05),whereas the expression levels of OPG,BALP,and IGF-1 were significantly reduced(P<0.05).Compared with the Model group,XFC treatment resulted in reduced expression levels of IL-1β,IL-6,TNF-α,TGF-β1,TRACP-5b,p-PI3K,and p-AKT(P<0.05),increased expression levels of OPG,BALP,and IGF-1(P<0.05),and alleviated cartilage damage.The SC79 group exhibited more severe inflammation,bone metabolism disorders,and cartilage damage compared with the Model group.Compared with the XFC-H group,co-administration of SC79 partially reversed the ameliorative effects of XFC-H on inflammation,bone metabolism disorder,and cartilage damage.Conclu-sion XFC alleviates inflammation and bone metabolism disorders in OA rats and ameliorates cartilage damage by regulating the PI3K/AKT signaling pathway.关键词
骨关节炎/新风胶囊/炎症/骨代谢/PI3K/AKT信号通路Key words
Osteoarthritis/Xinfeng Capsule(新风胶囊)/Inflammation/Bone metabolism/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
方大海,刘健,刘胜锋,程雪妮,张先恒,丁香,陈晓露..新风胶囊调节PI3K/AKT信号通路减轻骨关节炎大鼠炎症及骨代谢紊乱的机制研究[J].时珍国医国药,2026,37(3):417-423,7.基金项目
国家中医药管理局高水平中医药重点学科项目(ZYYZDXK-2023100) （ZYYZDXK-2023100）
现代中医内科应用基础与开发研究安徽省重点实验室项目(2016080503B041) （2016080503B041）
安徽省第12批"115"创新团队项目(皖人才办[2019]1号) （皖人才办[2019]1号）
