日用化学品科学2026，Vol.49Issue(3)：24-30,7.DOI:10.3969/j.issn.1006-7264.2026.03.004
虎杖中白藜芦醇提取工艺优化及其美白功效研究
Optimization of the resveratrol extraction process from polygonum cuspidatum and evaluation of its skin-whitening efficacy
张兴琪 1刘瑞学 1谈小芳 1牛强 2陈家享2
作者信息
- 1. 广东芭薇生物科技股份有限公司,广东 广州 510450
- 2. 广东悠质检测技术有限公司,广东 广州 510450
- 折叠
摘要
Abstract
Polygonum cuspidatum was used as the raw material to optimize the extraction and purification process for high-purity resveratrol.Different extraction methods,column chromatography resin types,ethanol concentrations for column elution,and ethanol concentrations for crystallization were systematically investigated.The skin-whitening efficacy of the purified resveratrol was evaluated using tyrosinase inhibition assays and zebrafish melanogenesis inhibition tests.The results demonstrated that the optimal conditions for the water-ethanol extraction and purification of high-content resveratrol were as follows:enzymatic hydrolysis-assisted stirring extraction as the extraction method,LXP-6 resin for column chromatography,70%ethanol as the column eluent,and 40%ethanol as the crystallization solvent.Under these optimized conditions,the average purity of resveratrol reached 99.13%.Evaluation of skin-whitening efficacy indicated that resveratrol strongly inhibited tyrosinase,with an IC₅₀ value of 8.58 μg/mL,markedly lower than kojic acid(19.08 μg/mL).In addition,treatment with 50 μg/mL resveratrol extract inhibited melanin production in zebrafish embryos by 23.76%,showing a statistically significant difference compared with the blank control group(P<0.05).These results indicate that resveratrol possesses potential skin-whitening effects through both tyrosinase inhibition and melanogenesis suppression pathways.关键词
白藜芦醇/虎杖/提取纯化/柱层析/美白功效/酶解Key words
resveratrol/polygonum cuspidatum/extraction and purification/column chromatography/skin-whitening efficacy/enzymatic hydrolysis分类
化学化工引用本文复制引用
张兴琪,刘瑞学,谈小芳,牛强,陈家享..虎杖中白藜芦醇提取工艺优化及其美白功效研究[J].日用化学品科学,2026,49(3):24-30,7.
