日用化学品科学2026，Vol.49Issue(3)：62-67,6.DOI:10.3969/j.issn.1006-7264.2026.03.009
桃胶缓解重金属所致皮肤成纤维细胞损伤的作用研究
Protective effects of peach gum on heavy metal-induced damage in human skin fibroblasts
邹娟 1方杨杨 2叶慧 2李医明 1李慧良 2郭夫江1
作者信息
- 1. 上海中医药大学 中药学院,上海 201203
- 2. 杭州花凝香生物科技有限公司,浙江 杭州 310000
- 折叠
摘要
Abstract
A heavy metal-induced human skin fibroblast(HFF-1)damage model was established using nickel sulfate(NiSO4).The protective effects of peach gum against heavy metal-induced cytotoxicity were evaluated through cell viability assays,morphological observation,and DNA damage marker(γ-H2AX)detection.Results demonstrated that exposure to NiSO4(8 mmol/L)for 12 h reduced HFF-1 cell viability to 44.65%(p<0.01)and significantly increased γ-H2AX expression in cells by 27.16-fold compared with the control.Pre-treatment with 8 mg/mL peach gum significantly improved cell viability to 59.22%(p<0.01)and decreased γ-H2AX expression to 3.8-fold of the control,indicating substantial protection against DNA damage.Component analysis revealed that the total sugar content of peach gum was 43.8%(phenol-sulfuric acid method).The polysaccharide fraction had an average molecular weight of 803.81 kDa(determined by high-performance liquid gel permeation chromatography)and was primarily composed of arabinose(47.96%),galactose(33.16%),xylose(13.41%),mannose(3.52%),and rhamnose(1.95%)as determined by gas chromatography-mass spectrometry(GC-MS).These findings suggest that the polysaccharide components in peach gum may alleviate heavy metal-induced cytotoxicity in skin fibroblasts,potentially through the mitigation of DNA damage mechanisms.关键词
桃胶/多糖/重金属/DNA损伤/皮肤保护Key words
peach gum/polysaccharides/heavy metals/DNA damage/skin protection分类
化学化工引用本文复制引用
邹娟,方杨杨,叶慧,李医明,李慧良,郭夫江..桃胶缓解重金属所致皮肤成纤维细胞损伤的作用研究[J].日用化学品科学,2026,49(3):62-67,6.
