无机材料学报2026，Vol.41Issue(2)：225-233,中插5-中插7,12.DOI:10.15541/jim20250169
多巴胺醌功能化的量子点荧光探针构建及其pH响应研究
Functionalized Quantum Dot Fluorescent Probes with Dopamine Quinone:Construction and pH Response
摘要
Abstract
Quantum dots(QDs)have demonstrated significant potential in the field of biological detection due to their excellent photoluminescence properties.As one of the key parameters in regulating physiological functions,pH response with high sensitivity is of great significance.However,conventional pH fluorescent probes are frequently limited by insufficient sensitivity or poor stability.In this work,band gap engineering was used to design and construct a CdSe/CdS/ZnS core-shell structured QD to improve the fluorescence quantum yield and stability.Additionally,the modification with mercaptoethylamine(MEA)and dopamine-isothiocyanate(DA-ITC)results in improved QD fluorescent probe exhibiting a highly sensitive pH response.Experimental results indicate that the amino-protected CdSe/CdS/ZnS QDs possess excellent optical properties.Following modification with DA-ITC,the probe exhibits highly sensitive pH response.The underlying response mechanism is attributed to fluorescence quenching effect of the dopamine quinone(DQ),which is formed by oxidation in surface ligands on the QDs under alkaline conditions.For 1 nmol QDs,when the input amount of DA-ITC is in the range of 4-40 μg/nmol,the fluorescence intensity of the probe reveals a linear decreasing trend with increasing pH from 5.0 to 10.0(R2>0.90).Notably,when the addition of DA-ITC is set at 20 μg/nmol,the probe demonstrates optimal pH responsiveness(R2=0.9869).Moreover,this probe has good cytocompatibility,allowing for effective application in fluorescence imaging for monitoring of cell pH.关键词
量子点/核壳结构/pH响应/氧化还原Key words
quantum dot/core-shell structure/pH response/redox分类
化学化工引用本文复制引用
王政,侯小琪,刘宣勇..多巴胺醌功能化的量子点荧光探针构建及其pH响应研究[J].无机材料学报,2026,41(2):225-233,中插5-中插7,12.基金项目
浙江省重点研发计划(2024C03080) （2024C03080）
国科大杭州高等研究院自主部署科研项目(2023HIAS-Y020)Key Research and Development Program of Zhejiang Province(2024C03080) （2023HIAS-Y020）
Research Funds of Hangzhou Institute for Advanced Study,UCAS(2023HIAS-Y020) （2023HIAS-Y020）
