新疆农业科学2025，Vol.62Issue(12)：2931-2940,10.DOI:10.6048/j.issn.1001-4330.0000.00.000
一株防治瓜列当的苍黄拟无枝酸菌GB5-8筛选与鉴定
Screening and identification of Amycolatopsis lurida GB5-8 against Phelipanche aegyptiaca
摘要
Abstract
[Objective]P.aegyptiaca is an all-parasitic weed,which mainly harms the melon crops and processed tomatoes in Xinjiang,resulting in a large amount of yield reduction,so it is important to select the microorganisms against the seed germination of the P.aegyptiaca for the biological control.To isolate biocontrol microorganisms with high inhibition on seed germination from the rhizosphere soil of processed tomato fields in Xinjiang,and their biocontrol effects were verified so as to provide biocontrol bacteria resources for the biological control of P.aegyptiaca in Xinjiang.[Methods]The actinomycetes were isolated from soil by gradient dilution method,and the strains with high inhibition on seed germination of P.aegyptiaca were screened by culture dish method.The strains were identified by biochemical and 16S rRNA gene sequencing technology,and the biocontrol effect of the strains on P.aegyptiaca was verified by pot experiment.[Results]70 strains of actinomycetes were isolated from the rhizosphere soil of the processed tomato field.The germination inhibition rates of GA6-2,GB3-1,GB3-2,GB5-7 and GB5-8 were 100%when the fermentation broth concentration was 1×106～1×109 CFU/mL.The fresh weight of tomato plants treated with GB5-8(12.86 g/plant)and GB5-7(11.56 g/plant)was significantly higher than those by other treatments,and the number of nodules of P.aegyptiaca in the roots of tomato plants treated with GB5-8(5.71 per plant)and GB5-7(5.88 per plant)was significantly lower than those by other treatments.The biomass of P.aegyptiaca with GB5-8(15.07 g)and GB3-1(15.68 g)was significantly lower than those by other treatments.Based on colony morphology,physiological and biochemical characteristics and phylogenetic tree analysis,GB5-8 was identified as A.lurida.[Conclusion]Amycolatopsis lurida GB5-8 can effectively inhibit the seed germination and reduce the parasite amount of P.aegyptiaca,which has the potential to control P.aegyptiaca.关键词
瓜列当/放线菌/苍黄拟无枝酸菌/生物防治Key words
Phelipanche aegyptiaca/actinomyces/Amycolatopsis lurida/biological control分类
农业科技引用本文复制引用
何伟,罗文芳,周军辉,黄欣,宋素琴,许建军..一株防治瓜列当的苍黄拟无枝酸菌GB5-8筛选与鉴定[J].新疆农业科学,2025,62(12):2931-2940,10.基金项目
新疆现代农业产业技术体系(XJARS-07) （XJARS-07）
新疆农业科学院农业科技创新稳定支持专项(xjnkywdzc-2022004) （xjnkywdzc-2022004）
