新疆农业科学2025，Vol.62Issue(12)：2941-2949,9.DOI:10.6048/j.issn.1001-4330.0000.00.000
库尔勒香梨黑斑病拮抗菌筛选、鉴定及评价
Screening,identification and evaluation of antagonistic bacteria against Kolra fragrant pear black spot pathogen
摘要
Abstract
[Objective]To isolate and screen out bacteria from the soil of a fragrant pear garden that possess inhibitory properties against Alternariaalternata.We also aim to identify the species and classification status of these bacteria and test their effectiveness in inhibiting Alternariaalternata.Evaluated in vitro the potential of these antagonists to control the disease caused by Alternaria alternata.[Methods]Using strain A923 of Alternaria alternata as the test pathogen,the antagonistic bacteria were isolated and screened by gradient dilution method and plate confrontation method.Meanwhile,the antagonistic species were identified by molecular data combined with morphological features,and their impact was evaluated against pear black spot by in vitro fruit evaluation experiment.[Results]15 bacterial strains were isolated,and the results of plate inhibition experiment showed that strains AW-28,24-HS-12,Shi233 and Shi213 had better inhibition effect on the tested pathogens.These strains were identified as Bacillus mobilis(OR835289),Bacillus albus(OR835281),Paenibacillusottowii(PP762202),and Pseudomonas chlororaphis(PQ241669)respectively.Strains AW-28(inhibitory rate 75.01%±2.07%)and 24-HS-12(inhibitory rate 73.37%±1.71%)with good bacteriostatic effect were further selected for in vitro fruit control test.The results showed that the control effect of spraying antagonistic fermentation solution for 7 days could reach the effects 43.82%and 32.58%.[Conclusion]Strains AW-28 and 24-HS-12 have certain control potential,which provides a theoretical basis and potential bacterial biocontrol resources for the subsequent biological control of this disease.关键词
库尔勒香梨/黑斑病/拮抗菌/鉴定/生物防治Key words
Korla Fragrant Pear/black spot/antagonistic bacteria/identification/biological control分类
农业科技引用本文复制引用
宋博,徐兵强,张丽娟,何天明,艾米都拉·克尤木,魏增宙,孙一鸣,朱晓锋..库尔勒香梨黑斑病拮抗菌筛选、鉴定及评价[J].新疆农业科学,2025,62(12):2941-2949,9.基金项目
农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室开放基金资助课题(KFJJ20160103) （KFJJ20160103）
植物病虫害生物学国家重点实验室开放基金资助课题(SKLOF202207) The Open Fund Project for Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis,Ministry of Agriculture and Rural Affairs(KFJJ20160103) （SKLOF202207）
The Open Fund Project of State Key Laboratory for Biology of Plant Diseases and Insect Pests(SKLOF202207) （SKLOF202207）
