药食同源杂志2025，Vol.1Issue(9)：67-73,7.
不同产地茶叶中咖啡因、茶多酚含量及微量元素的分析
Content of Caffeine,tea polyphenols,and trace element in tea leaves from different producing areas
摘要
Abstract
Objective To establish a multidimensional analysis system for comparing the differences in caffeine,tea polyphenol content,and trace element composition among tea leaves from different cultivars and growth stages,thereby providing a scientific basis for tea quality evaluation and clinical applications.Method Two tea samples,namely Jinjing black tea and Anhua black tea,along with six samples of Chengbu Dong tea collected between 2019 and 2024,were selected as the primary research subjects.High-performance liquid chromatography(HPLC),ultraviolet-visible spectrophotometry(UV-Vis),and inductively coupled plasma mass spectrometry(ICP-MS)were employed to quantify the content of caffeine,tea polyphenols,and trace elements,respectively.Results The caffeine content in the eight tea samples ranged from 2.60%to 3.82%,with Chengbu Dong tea from 2023 exhibiting the highest level(3.82%).A fluctuating decline was observed from 2019 to 2022.The tea polyphenol content ranged from 20.19%to 31.05%,reaching a peak in Chengbu Dong tea from 2024(26.13%),which surpassed the range observed from 2019 to 2023(20.19%–24.25%)and marked the highest value in six years,with a notable increase.The primary trace elements,including manganese(244,633–563,168 ppb),copper(8,436–17,682 ppb),strontium(4,474–13,286 ppb),and nickel(3,413–14,376 ppb),were present at relatively high levels.Notably,the manganese content in Chengbu Dong tea from 2021 was particularly prominent(479,394 ppb),and all heavy metal content complied with relevant standards.Conclusion Significant differences exist in the caffeine and tea polyphenol content among tea leaves from different cultivars and growth stages.Chengbu Dong tea generally exhibits higher caffeine content,with slight variations in tea polyphenol content across different growth stages.The heavy metal content in Chengbu Dong tea meet regulatory requirements,while the concentrations of various trace elements demonstrate specificity,likely associated with soil enrichment or cultivar characteristics.This study provides data support for the precise utilization of tea resources and the development of functional tea products.关键词
茶叶/茶多酚/咖啡因/微量元素/高效液相色谱法/紫外分光光度法Key words
tea leaves/tea polyphenols/caffeine/trace elements/high-performance liquid chromatography/ultraviolet-visible spectrophotometry分类
医药卫生引用本文复制引用
李强,肖敦旺,肖娟,张新,陈雨迪,索俪榕..不同产地茶叶中咖啡因、茶多酚含量及微量元素的分析[J].药食同源杂志,2025,1(9):67-73,7.基金项目
2019年城步苗族自治县重点产业扶贫项目(湘财预[2019]73号). （湘财预[2019]73号）
