西北农林科技大学学报（自然科学版）2026，Vol.54Issue(3)：35-45,11.DOI:10.13207/j.jnwafu.2026.03.004
余甘子转录组与SSR信息分析及分子标记开发
Analysis of Phyllanthus emblica transcriptome and SSR information and molecular marker development
摘要
Abstract
[Objective]This research aims to clarify the distribution characteristics of SSR loci in the transcriptome of Phyllanthus emblica and develop the SSR molecular marker technology of P.emblica,to provide a theoretical basis for the identification of genetic relationships,genetic breeding,and the innovative utilization of P.emblica.[Method]Six leaf samples of P.emblica exhibiting significant phenotypic diffe-rences were utilized as materials.Non-parametric transcriptome data for these leaves were obtained through high-throughput sequencing technology,followed by an analysis of the transcriptome and SSR locus chara-cteristics.SSR primers were then designed and evaluated for their effectiveness and polymorphism.[Re-sult]The results indicated that a total of 65 074 genes were identified through transcriptome sequencing of P.emblica leaves,with an average length of 1 064 bp.Among them,71.77%of the genes were successfully annotated.Additionally,a total of 18 245 SSR loci were identified across all genes,showing a frequency of 21.68%,which included 192 repeat motifs,with A/T repeat motifs being the most abundant.Among all lo-ci,2 714 polymorphic SSRs were detected,accounting for 14.88%,suggesting that SSR loci had great po-tential for genetic diversity analysis.A total of 12 488 pairs of SSR primers were su-ccessfully designed for the 18 245 genes containing SSR loci,with a success rate of 73.88%.Among all the primers,7 602 pairs of primers had the repeat motif of mononucleotide,accounting for 60.87%.Subsequently,154 pairs of primers were randomly selected for validation and polymorphism analysis.Ultimately,10 pairs of SSR primers ex-hibiting clear amplified bands and good polymorphism were identified.The repeat motifs of these 10 pairs consisted of 7 dinucleotides,1 trinucleotide,and 2 pentanucleotides.The size of the amplified products ranged from 127 to 270 bp,with an average of 220.5 bp.[Conclusion]Polymorphic SSR loci hold signifi-cant potential for genetic diversity.10 pairs of SSR primers,which exhibited clear amplified bands and sub-stantial polymorphism were selected.关键词
余甘子/转录组/SSR/引物筛选/分子标记Key words
Phyllanthus emblica/transcriptome/SSR/primers screening/molecular marker分类
农业科技引用本文复制引用
王建超,张小艳,谢丽雪,张立杰,李韬..余甘子转录组与SSR信息分析及分子标记开发[J].西北农林科技大学学报（自然科学版）,2026,54(3):35-45,11.基金项目
国家热带植物种质资源库-余甘子种质资源分库项目(NTPGRC2024-011) （NTPGRC2024-011）
福建省属公益类科研院所专项(2022R1028004,2024R1027002) （2022R1028004,2024R1027002）
福建省人民政府与中国农业科学院农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021019-GSS01) （XTCXGC2021019-GSS01）
