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RGS14通过抑制TAK1改善急性心肌梗死的作用及机制研究

吴清华段绍锋谈重愿张丽荣张家兴胡宇峰折志刚李红良

医学新知2026，Vol.36Issue(2)：169-178,10.

医学新知2026，Vol.36Issue(2)：169-178,10.DOI:10.12173/j.issn.1004-5511.202511095

RGS14通过抑制TAK1改善急性心肌梗死的作用及机制研究

Role and mechanism of RGS14 in improving acute myocardial infarction by inhibiting TAK1 activation

吴清华 ¹段绍锋 ²谈重愿 ²张丽荣 ²张家兴 ²胡宇峰 ²折志刚 ³李红良³

作者信息

1. 武汉大学人民医院心血管内科(武汉 430060)||重大疾病新药靶发现及新药创制全国重点实验室(江西赣州 341000)
2. 重大疾病新药靶发现及新药创制全国重点实验室(江西赣州 341000)||赣南医科大学赣南创新与转化医学研究院(江西赣州 341000)
3. 武汉大学人民医院心血管内科(武汉 430060)||重大疾病新药靶发现及新药创制全国重点实验室(江西赣州 341000)||赣南医科大学赣南创新与转化医学研究院(江西赣州 341000)
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摘要

Abstract

Objective This study aims to investigate the role and mechanism of regulator of G-protein signaling 14(RGS14)protein in acute myocardial infarction(AMI).Methods The RGS14 knockout mice were selected to construct AMI model.The cardiac tissue and serum of mice were collected 24 h after modeling,the degree of inflammatory infiltration in the cardiac tissue was analyzed by immunohistochemical analysis,and biochemical analyzer detects serum biochemical indicators.Overexpression and knockdown adenovirus were constructed in vitro and transfected into primary rat cardiomyocytes.RT-qPCR and Western blot were used to detect the expression of RGS14,inflammation and apoptosis related markers mRNA and protein.TUNEL staining was used to analyze the apoptosis of heart tissue.Results The expression of RGS14 in myocardial cells of AMI model mouse was downregulated.Compared with wild-type mice,RGS14 knockout mice exhibited increased AMI induced cardiac dysfunction,inflammatory response,and apoptosis.Conversely,overexpression of RGS14 inhibited cardiomyocyte apoptosis in vitro.The protective effect of RGS14 on AMI injury is closely related to its inhibition of TAK1-JNK/p38 signaling pathway activation.Inhibition of TAK1 alleviates the adverse effects of RGS14 knockdown on AMI in vitro.Conclusion RGS14 exerts a protective function in the process of inflammation and apoptosis after AMI,and alleviates cardiac dysfunction by inhibiting the activation of TAK1,which provides a new potential for the treatment of AMI.

关键词

RGS14/急性心肌梗死/炎症/细胞凋亡/TAK1

Key words

Regulator of G-protein signaling 14/Acute myocardial infarction/Inflammation/Apoptosis/TAK1

分类

医药卫生

引用本文复制引用

吴清华,段绍锋,谈重愿,张丽荣,张家兴,胡宇峰,折志刚,李红良..RGS14通过抑制TAK1改善急性心肌梗死的作用及机制研究[J].医学新知,2026,36(2):169-178,10.

基金项目

国家自然科学基金面上项目(82570452,82270390) （82570452,82270390）

江西省自然科学基金重大项目(20252BAC270001) （20252BAC270001）

医学新知

ISSN：1004-5511

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