医药导报2026，Vol.45Issue(3)：383-389,7.DOI:10.3870/j.issn.1004-0781.2026.03.004
鳖甲活性肽抗肝癌作用网络药理学探讨及体外活性验证
Network Pharmacological Investigation and In Vitro Activity Validation of the Anti-Hepatocellular Carcinoma Effect of the Active Peptides from Hydrolysates of Turtle Shell Protein
摘要
Abstract
Objective Anti-hepatocellular carcinoma mechanism of active peptides derived from hydrolyzates of turtle shell protein(HTSP)was elucidated by employing network pharmacology,molecular docking,and in vitro assays.Methods The study collected two active peptides from the hydrolysates of turtle shell protein(HTSP),namely HTSP-9 and HTSP-11,which were previously identified by our research group.The potential targets of these active peptides were predicted using the SuperPred and Swiss Target Prediction websites.Subsequently,the HCC-related targets were screened through databases including Gene-Cards,DisGeNET,and OMIM to identify the overlapping targets between the two peptides.Subsequently,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were conducted on the overlapping targets.Cy-toscape software was used to construct the HTSP-HCC-Overlapping Targets protein-protein interaction network(PPI)and screen core targets.Molecular docking experiments were performed on the HTSP and the core targets of action using AutoDock software,and finally,the network pharmacology findings were corroborated by in vitro cellular assays.Results The analysis yielded 37 core targets shared by the HTSP and HCC.The KEGG enrichment analysis involves signaling pathways such as PI3K-Akt,TNF,and apoptosis.In vitro experiments demonstrated that the HTSP could inhibit the proliferation of HepG2 cell.Conclusion The HTSPs are likely to exert therapeutic effects against HCC by modulating the growth and differentiation of hepatocellular carcinoma HepG2 cells through a multi-target and multi-pathway approach.关键词
鳖甲活性肽/肝细胞癌/网络药理学/分子对接Key words
Hydrolysates of turtle shell protein/Hepatocellular carcinoma/Network pharmacology/Molecular docking分类
医药卫生引用本文复制引用
黄雅宁,尤朋涛,胡俊杰,苟君波,田娟,张剑锋,胡春玲..鳖甲活性肽抗肝癌作用网络药理学探讨及体外活性验证[J].医药导报,2026,45(3):383-389,7.基金项目
湖北省药品监督检验研究院重点实验室开放课题项目(2023HBKFZ002) （2023HBKFZ002）
湖北中医药大学重大科技攻关项目(2023ZDXM007) （2023ZDXM007）
中药资源与中药化学湖北省重点实验室开放基金(KLRCCM2405) （KLRCCM2405）
荆楚理工学院药物合成与优化湖北省重点实验室(YB202302). （YB202302）
