医药导报2026，Vol.45Issue(3)：389-395,7.DOI:10.3870/j.issn.1004-0781.2026.03.005
固相萃取-高效液相色谱法测定盐附子中乌头碱含量及大鼠血浆附子类生物碱浓度
Determination of Aconite Alkaloids in Yan Fuzi and Rat Plasma by Solid Phase Extraction Coupled with High Performance Liquid Chromatography
摘要
Abstract
Objective To establish a solid phase extraction-high performance liquid chromatography(SPE-HPLC)method for the rapid determination of aconitine,mesaconitine,hypaconitine,lappaconitine,and benzoylaconine in salt-processed Aconiti Lateralis Radix Praeparata(Yan Fuzi)and rat plasma 30 min after intragastric administration of a Yan Fuzi decoction.Methods Zeolitic imidazolate framework-8(ZIF-8)material was synthesized and characterized according to literature methods.By optimizing the preparation process of metal-organic frameworks and solid-phase extraction conditions,a quantitative analytical method coupling extraction with liquid chromatography was developed.This method was applied to detect the plasma concentrations of aconitine,hypaconitine,mesaconitine,lappaconitine,and benzoylaconine in rat plasma following intragastric administration of Yan Fuzi decoction(0.375 g·kg-1 body weight).Results The contents of five aconite alkaloids in Yan Fuzi were determined as follows:aconitine 62.78 mg·kg-1,mesaconitine 500.4 mg·kg-1,hypaconitine 37.80 mg·kg-1,lappaconitine 0.144 mg·kg-1,and benzoylaconine 36.14 mg·kg-1.In plasma samples collected 30 min post-administration,the concentra-tions detected were:aconitine 2.06 μg·mL-1,mesaconitine 11.79 μg·mL-1,hypaconitine 1.69 μg·mL-1,lappaconitine 1.77 μg·mL-1,and benzoylaconine 23.22 μg·mL-1.Method validation confirmed that all parameters met quantitative determi-nation requirements.Conclusion The established method enables accurate quantification of aconite alkaloids in both Aconiti Lateralis Radix Praeparata and plasma samples after oral administration,providing a valuable reference for clinical monitoring of these compounds in biological matrices.关键词
盐附子/沸石咪唑酯骨架材料/附子类生物碱/固相萃取-高效液相色谱法Key words
Salt processed Aconiti Lateralis Radix Praeparata(Yan Fuzi)/Zeolitic imidazolate framework material/Ac-onitum alkaloids/Solid-phase extraction coupled with high performance liquid chromatography分类
医药卫生引用本文复制引用
刘仁炎,朱军,杨洋,冯宝栋,明庭文,辛灵怡,黄嘉欣,绉红海,刘慧敏,陈琴华..固相萃取-高效液相色谱法测定盐附子中乌头碱含量及大鼠血浆附子类生物碱浓度[J].医药导报,2026,45(3):389-395,7.基金项目
武当特色中药研究湖北省重点实验室(湖北医药学院)开放课题(WDCM2024008) （湖北医药学院）
深圳医疗卫生三名工程(No.SZZYSM202106004) （No.SZZYSM202106004）
十堰市科技局引导性项目(24Y152) （24Y152）
国药东风总医院创新团队项目(2024C07) （2024C07）
国药东风总医院新技术新业务项目(SX20250203). （SX20250203）
