医药导报2026，Vol.45Issue(3)：402-409,8.DOI:10.3870/j.issn.1004-0781.2026.03.007
四君子汤对结肠病变小鼠模型的作用机制
Mechanistic Study of Sijunzi Decoction on Colonic Lesions in Mice
摘要
Abstract
Objective To investigate the therapeutic effects of Sijunzi decoction on dextran sulfate sodium(DSS)-in-duced colonic lesions in mice.Methods The aqueous extract of Sijunzi decoction was prepared via water decoction,and its pol-ysaccharides were isolated using ethanol precipitation and the Sevag method.The purity,molecular weight,and monosaccharide composition of the polysaccharides were analyzed via high-performance liquid gel chromatography and gas chromatography-mass spectrometry(GC-MS).Mice were divided into normal,model,positive drug control(mesalazine,0.1 g·kg-1),aqueous extract(5 g·kg-1 and 15 g·kg-1,based on raw herb weight),and polysaccharide(0.18 g·kg-1 and 0.53 g·kg-1,equivalent to raw herb doses of 5 g·kg-1 and 15 g·kg-1)groups.Except for the normal group,modeling mice were administered 3％DSS in drink-ing water for 7 days to induce colitis(with concurrent oral treatments).Disease Activity Index(DAI)scores were assessed on day 4.On day 8,animals were anesthetized and sacrificed,and their colon length,mass,mass-to-length ratio,macroscopic and his-topathological injury scores were measured.Immunohistochemistry was used to evaluate colonic protein expression levels.Results Sijunzi decoction polysaccharides were heteropolysaccharides with a relative molecular mass of approximately 4881 Da,com-posed of glucose(79.06％),galactose(9.49％),arabinose(7.37％),and minor monosaccharides.Both the aqueous extract and polysaccharides reduced DAI scores,macroscopic and histopathological damage,alleviated colon shortening and weight gain,and restored the decreased expression of colonic HuR,E-cadherin,Rac1,and Cdc42 proteins induced by DSS.Conclusion The aqueous extract and polysaccharides of Sijunzi decoction exert protective effects against DSS-induced colonic lesions in mice,likely mediated by upregulating colonic HuR,E-cadherin,Rac1,and Cdc42 protein expression.关键词
四君子汤/多糖/葡聚糖硫酸钠/结肠病变Key words
Sijunzi decoction/Polysaccharides/Dextran sulfate sodium/Colonic lesions分类
医药卫生引用本文复制引用
李志锦,朱泽明,戴杏珍,陈婉霞,张东,胡玲,李茹柳..四君子汤对结肠病变小鼠模型的作用机制[J].医药导报,2026,45(3):402-409,8.基金项目
国家自然科学基金资助项目(81673940) （81673940）
广州中医药大学"一流学科研究重点项目"(广中医校办[2020]62号). （广中医校办[2020]62号）
