针刺研究2026，Vol.51Issue(3)：294-301,8.DOI:10.13702/j.1000-0607.20250234
电针对帕金森病小鼠中脑黑质SIRT3/NF-κB/MAPK信号通路的影响
Effects of electroacupuncture on the SIRT3/NF-κB/MAPK signaling pathway in the substantia nigra of mice with Parkinson's disease
摘要
Abstract
Objective To investigate the effects of electroacupuncture(EA)at"Fengfu"(GV16)and"Taichong"(LR3)on tyrosine hydroxylase(TH),microglia(MG),and the silent information regulator 3(SIRT3)/nuclear factor-kappa B(NF-κB)/mitogen-activated protein kinase(MAPK)signaling pathway-related proteins in the substantia nigra of Parkinson's disease(PD)mice,and to explore the potential mechanisms underlying the therapeutic effects of EA on PD.Methods C57BL/6J mice were randomly divided into 4 groups:control group,model group,non-acupoint group,and EA group,with 12 mice in each group.A PD model was established by intraperitoneal injections of MPTP for 7 consecutive days.The EA group received EA at GV16 and LR3.The non-acupoint group underwent needle insertion 3 mm lateral to GV16 and LR3 without electrical stimulation.Treatments were administered for 15 min per session,once daily for 14 days.Motor function was assessed using the pole test and suspension test.Immunohistochemistry was used to detect TH-positive expression in the substantia nigra.Immunofluorescence was employed to quantify activated MG.Western blot was used to measure the protein expression levels of SIRT3,NF-κB p65,and p38 MAPK.ELISA was used to determine the serum levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β).Results Compared with the control group,the model group exhibited a longer total time in the pole test(P<0.01),a lower score in the suspension test(P<0.01),a reduce in mean TH optical density in the substantia nigra(P<0.01),an increase in the number of MG-positive cells(P<0.01),a decrease in SIRT3 protein expression(P<0.01).The protein expression levels of NF-κB p65 and p38 MAPK proteins in the substantia nigra,and the serum levels of TNF-α,and IL-1β were all elevated(P<0.01).Compared with model group and the non-acupoint group,the EA group showed a shortened total time in the pole test(P<0.01),a higher score in the suspension test(P<0.01),an increase in mean TH optical density in the substantia nigra(P<0.01),a decrease in the number of MG-positive cells(P<0.01),an increase in SIRT3 protein expression(P<0.05).The expression levels of NF-κB p65 and p38 MAPK proteins in the substantia nigra,and the serum contents of TNF-α and IL-1β were all decreased(P<0.01).Conclusion EA can improve motor dysfunction in PD mice,reduce the loss of dopaminergic neurons,and increase TH expression in the substantia nigra.The underlying mechanism may involve the modulation of the SIRT3/NF-κB/MAPK signaling pathway to suppress neuroinflammation.关键词
帕金森病/电针/小胶质细胞/沉默信息调节因子3/核因子κB/丝裂原活化蛋白激酶Key words
Parkinson's disease/Electroacupuncture/Microglia/Silent information regulator 3/Nuclear factor-kappa B/Mitogen-activated protein kinase引用本文复制引用
祁羚,张小蕾,李亚楠,汪瑶,李含章,肖蝶,陶晓亿,马骏..电针对帕金森病小鼠中脑黑质SIRT3/NF-κB/MAPK信号通路的影响[J].针刺研究,2026,51(3):294-301,8.基金项目
国家自然科学基金项目(No.81473788) （No.81473788）
湖北省联合基金项目(No.2024AFD305) （No.2024AFD305）
武汉市科技局知识创新专项(No.2023020201020794) （No.2023020201020794）
