针刺研究2026，Vol.51Issue(3)：302-309,8.DOI:10.13702/j.1000-0607.20250170
基于NRF2/GPX4通路探讨益元灸调控铁死亡改善脊髓损伤大鼠尿潴留的机制
Effect of Yiyuan moxibustion on regulating ferroptosis in rats with urinary retention after spinal cord injury via the NRF2/GPX4 pathway
摘要
Abstract
Objective To investigate the mechanism of Yiyuan moxibustion in improving lipid peroxidation and urine retention in spinal cord injury(SCI)rats by regulating ferroptosis via the nuclear factor erythroid 2-related factor 2(NRF2)/glutathione peroxidase 4(GPX4)pathway.Methods Forty-eight female Wistar rats were randomly divided into sham operation,model,moxibustion and moxibustion+inhibitor(combination)groups,with 12 rats in each group.SCI induced urinary retention model was established by using Allen's method.Moxibustion was applied to"Shenque"(CV8),"Guanyuan"(CV4)and"Zhongji"(CV3)for 15 min,once daily for 14 d.Rats in the combination group received moxibustion combined with intraperitoneal injection of the NRF2 inhibitor ML385(30 mg/kg)once daily for 14 consecutive days.Urodynamic analysis was used to record bladder leak point pressure,maximum bladder volume,and bladder compliance to evaluate bladder function.H.E.staining was used to observe morphological changes in spinal cord tissue.Prussian blue staining was used to observe iron deposition in spinal cord tissue.Transmission electron microscopy(TEM)was used to observe mitochondrial morphological changes in spinal cord tissue.Colorimetric assays were used to measure serum Fe2+,malondialdehyde(MDA),and reduced glutathione(GSH)levels.Western blot was used to detect the protein expressions of NRF2,GPX4,and solute carrier family 7 member 11(SLC7A11)in spinal cord tissue.Results Rats in the model group had disrupted spinal architecture,vacuolated neurons,swollen mitochondria with lost cristae,and brownish iron deposits,which were relatively milder in the moxibustion group.Compared with the sham operation group,the bladder leak point pressure,serum GSH content,and expression levels of NRF2,SLC7A11,and GPX4 proteins in spinal cord tissue were all significantly reduced(P<0.01),while the maximum bladder volume and bladder compliance,as well as the serum contents of Fe2+and MDA,were significantly increased(P<0.01)in the model group.When compared to the model group and the combination group,the moxibustion group exhibited a decrease in maximum bladder volume,bladder compliance,serum Fe2+and MDA content(P<0.01),but an increase in bladder leak point pressure,GSH content,and the expression of NRF2,SLC7A11,and GPX4 proteins(P<0.01,P<0.05).Conclusion Yiyuan moxibustion may alleviate lipid peroxidation caused by ferroptosis in spinal neurons by up-regulating the NRF2/GPX4 pathway,thereby promoting the recovery of neural regulation of bladder function and improving urine retention in SCI rats.关键词
脊髓损伤/益元灸/铁死亡/脂质过氧化Key words
Spinal cord injury/Yiyuan moxibustion/Ferroptosis/Lipid peroxidation引用本文复制引用
王慧欣,段淑婷,柏承婕,高静,刘承梅..基于NRF2/GPX4通路探讨益元灸调控铁死亡改善脊髓损伤大鼠尿潴留的机制[J].针刺研究,2026,51(3):302-309,8.基金项目
国家自然科学基金面上项目(No.82474613) （No.82474613）
2023年度河南省中医学"双一流"创建科学研究专项(No.HSRP-DFCTCM-2023-2-22) （No.HSRP-DFCTCM-2023-2-22）
2023年度河南省卫生健康委国家中医药传承创新中心科研专项(No.2023ZXZX1037) （No.2023ZXZX1037）
2023年河南省中医药科学研究专项普通项目(No.2023ZY3003) （No.2023ZY3003）
