中国农业大学学报2026，Vol.31Issue(3)：89-96,8.DOI:10.11841/j.issn.1007-4333.2026.03.08
垂穗披碱草近壳多胞叶斑病快速检测技术的建立与应用
Establishment and application of molecular detection for Parastagonospora nodorum-induced leaf spot disease in Elymus nutans
摘要
Abstract
To establish a rapid detection system for leaf spot disease of Elymus nutans caused by Parastagonospora nodorum,highly specific primers Pr-F/Pr-R targeting the conserved RPB2(RNA polymerase Ⅱ subunit 2)gene sequence of the pathogen was designed.An efficient,rapid,and stable PCR molecular detection technique was developed to identify Parastagonospora leaf spot in E.nutans.The results indicated:1)The detection system specifically amplified a 127 bp band from P.nodorum,and the sensitivity of Pr-F/Pr-R for detecting genomic DNA of P.nodorum was 100 pg/μL.2)The Pr-F/Pr-R primers can specifically detect P.nodorum in infected E.nutans leaf tissues,as well as in suspected diseased field-collected E.nutans plants.These results provide a technical basis for the early detection and targeted control of leaf spot disease in E.nutans.关键词
垂穗披碱草/颖枯近壳多胞菌/叶斑病/分子检测Key words
Elymus nutans/Parastagonospora nodorum/leaf spot disease/molecular detection分类
农业科技引用本文复制引用
龙嘉惠,薛龙海,李春杰,朱永群..垂穗披碱草近壳多胞叶斑病快速检测技术的建立与应用[J].中国农业大学学报,2026,31(3):89-96,8.基金项目
国家重点研发计划项目(2022YFD1401101) （2022YFD1401101）
"双一流"建设经费-队伍建设经费-科研启动经费项目(561120201) （561120201）
国家农业产业技术体系项目(CARS-34) （CARS-34）
