中国水稻科学2026，Vol.40Issue(2)：244-252,9.DOI:10.16819/j.1001-7216.2026.250204
水稻野败型三系杂交种纯度检测功能标记的开发与应用
Development and Application of Functional Markers for Purity Identification of Wild-abortive Three-line Hybrid Rice
摘要
Abstract
[Objective]Developing stable,efficient,and reliable markers for purity testing of three-line hybrid seeds is crucial for current hybrid rice seed production.[Method]Based on the specific sequences of the wild abortive-type sterility gene WA352,the major fertility-restoring gene Rf4,and the internal reference gene Actin,multiple single-gene detection markers were designed.PCR amplification was then performed by optimizing the primer mixture ratio.[Result]A mixture of primers WA352-2,Rf4-3,and Actin-3 in a ratio of 1:11:3 enabled simultaneous detection of the genotypes of WA352,Rf4,and Actin in a single PCR reaction,allowing differentiation among wild abortive-type CMS lines,restorer lines,maintainer lines,and hybrids.[Conclusion]The detection method established in this study is simple to perform and yields stable and reliable results.It can not only identify genotypes in wild-abortive three-line rice but also serve as an effective tool for molecular marker-assisted breeding.By improving the accuracy of purity assessment during breeding,this method provides strong support for achieving high-yield and high-quality rice breeding objectives in China.关键词
水稻/野败/三系/检测标记/PCRKey words
rice/wild-abortive/three-line/detection marker/PCR引用本文复制引用
张梦柯,陆佳雨,何金,许学,吴爽,王沛然,陈若凡,金青,汪秀峰..水稻野败型三系杂交种纯度检测功能标记的开发与应用[J].中国水稻科学,2026,40(2):244-252,9.基金项目
安徽省科技攻坚计划项目(重大项目,202423m10050002) （重大项目,202423m10050002）
水稻种质创新与分子改良安徽省重点实验室开放基金资助项目(SDKF-2024-05). （SDKF-2024-05）
