中国免疫学杂志2026，Vol.42Issue(3)：541-546,6.DOI:10.3969/j.issn.1000-484X.2026.03.005
橙皮苷抑制ROS/NLRP3通路对肺炎链球菌感染的肺泡上皮细胞损伤的影响
Effect of hesperidin on alveolar epithelial cell damage caused by Streptococcus pneumoniae infection by inhibiting ROS/NLRP3 pathway
摘要
Abstract
Objective:To investigate effect of hesperidin on alveolar epithelial cell damage caused by Streptococcus pneumoniae infection by inhibiting reactive oxygen species(ROS)/nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)pathway.Methods:A549 alveolar epithelial cells were assigned into control group(untreated),infection group(1×108 CFU/mL Strep-tococcus pneumoniae stimulated cells),infection+low,medium and high-doses hesperidin groups(treated with 30,60,and 90 µmol/L hesperidin after infection),and infection+high-dose hesperidin+pathway activator trimethylamine N-oxide(TMAO)group(treated with 90 µmol/L hesperidin and 200 µmol/L TMAO after infection).CCK-8 assay was used to detect cell viability.Flow cytometry was applied to analyze cell apoptosis.ELISA was applied to detect levels of cytokines TNF-α,IL-1β and IL-6 in cells.DCFH-DA fluores-cent probe was applied to detect cellular ROS level.Reagent kit was used to detect MDA content and CAT,SOD activities in cells.Western blot was applied to analyze levels of Ki67,caspase-3,cyclooxygenase-2(Cox-2),nicotinamide adenine dinucleotide phos-phate oxidase(Nox)and NLRP3 proteins in cells.Results:Compared with control group,A450 value,CAT and SOD activities,and Ki67 protein level in infection group were decreased(P<0.05),while apoptosis rate,TNF-α,IL-1β,IL-6 and MDA levels,ROS fluo-rescence intensity,caspase-3,Nox and NLRP3 protein levels were increased(P<0.05).Compared with infection group,A450 value,CAT and SOD activities,and Ki67 protein level in infection+low/medium/high-dose hesperidin groups were increased(P<0.05),while apoptosis rate,TNF-α,IL-1β,IL-6 and MDA levels,ROS fluorescence intensity,caspase-3,Nox and NLRP3 protein levels were decreased(P<0.05).Compared with infection+high-dose hesperidin group,A450 value,CAT and SOD activities and Ki67 protein level ininfection+high-dose hesperidin+TMAO group were decreased(P<0.05),while apoptosis rate,TNF-α,IL-1β,IL-6 and MDA levels,ROS fluorescence intensity,caspase-3,Nox and NLRP3 protein levels were increased(P<0.05).Conclusion:Hesperidin may alleviate alveolar epithelial cell damage caused by Streptococcus pneumoniae infection by inhibiting ROS/NLRP3 pathway.关键词
橙皮苷/活性氧/核苷酸结合寡聚化结构域样受体蛋白3/肺炎链球菌/肺泡上皮细胞损伤Key words
Hesperidin/Reactive oxygen species/Nucleotide-binding oligomerization domain-like receptor protein 3/Strepto-coccus pneumoniae/Alveolar epithelial cell damage分类
医药卫生引用本文复制引用
黄文静,杨向琪,张艳敏,任乾,曾晓兵..橙皮苷抑制ROS/NLRP3通路对肺炎链球菌感染的肺泡上皮细胞损伤的影响[J].中国免疫学杂志,2026,42(3):541-546,6.基金项目
甘肃省科技计划项目(21JR1RG311) （21JR1RG311）
2023年甘肃省卫生健康行业科研项目(GSWSQN2023-19). （GSWSQN2023-19）
