中国免疫学杂志2026，Vol.42Issue(3)：574-580,7.DOI:10.3969/j.issn.1000-484X.2026.03.010
HLA-A/B基因多态性与女性高危型HPV16/18感染和宫颈癌易感性的关联性分析
Association analysis of HLA-A/B gene polymorphism with high-risk HPV16/18 infection and susceptibility to cervical cancer in women
摘要
Abstract
Objective:To investigate correlation between HLA-A/B gene polymorphisms in cervical cancer patients and susceptibility to high-risk HPV16/18 infection and cervical cancer.Methods:Observation group(n=205)consisted of cervical cancer patients diagnosed at the First Affiliated Hospital of Guangxi Medical University from January 2020 to June 2023,while control group(n=97)comprised healthy women participating in hospital's cervical cancer screening program during same period.PCR and sequenc-ing methods were employed to detect HLA-A/B gene typing in subjects;PCR-fluorescence labeling was used to detect HPV gene typing in subjects;level of CD8+T cells and positive rate of PD-1 and TIM-3,which inhibit surface activity,were detected by flow cy-tometry;Spearman correlation analysis was conducted to assess relationship between HLA-A/B gene polymorphisms and susceptibility to high-risk HPV infection,cervical cancer and CD8+T cell function in patients.Results:Among all subjects,11 HLA-A alleles and 22 HLA-B alleles were detected.Frequencies of HLA-A*02,A*03,A*11,HLA-B*27 and B*44 alleles were significantly higher in observation group compared to control group(all P<0.05).In cervical cancer patients with high-risk HPV infection,frequencies of HLA-A*02,A*11,HLA-B*07 and B*44 alleles were higher than patients with non-high-risk HPV infection(all P<0.05).Spearman correlation analysis indicated that HLA-A*02 and HLA-A*11 were positively correlated with cervical cancer(r=0.205,0.138;P=0.013,0.027),while HLA-A*02 and HLA-B*44 were positively correlated with high-risk and non-high-risk HPV infections,respec-tively(r=0.123,0.253;P=0.008,0.020).HLA-A*02,HLA-A*11 and HLA-B*44 were associated with level of CD8+T cells(r=0.115,0.169,0.153,P=0.011,0.025,0.020),positive rate of PD-1(r=0.101,0.136,0.163,P=0.040,0.033,0.031)and positive rate of TIM-3(r=0.124,0.201,0.211,P=0.005,0.008,0.009)were positively correlated.Conclusion:Carriers of HLA-A02 and A11 are significantly positively associated with high-risk HPV16/18 infection.HLA-A*02 and HLA-B*44 are susceptibility genes for cervical cancer in women,these may be related to depletion of CD8+T cell immune function.关键词
宫颈癌/HLA-A/B/易感性/高危型HPV/基因多态性Key words
Cervical cancer/HLA-A/B/Susceptibility/High-risk HPV/Genetic polymorphism分类
医药卫生引用本文复制引用
卢庭婷,黄海鹏,陈基强,张柯媛..HLA-A/B基因多态性与女性高危型HPV16/18感染和宫颈癌易感性的关联性分析[J].中国免疫学杂志,2026,42(3):574-580,7.基金项目
2023年度广西高校中青年教师科研基础能力提升项目(2023KY1413). （2023KY1413）
