中国比较医学杂志2026，Vol.36Issue(3)：83-92,10.DOI:10.3969/j.issn.1671-7856.2026.03.007
咪喹莫特与环磷酰胺诱导C57BL/6J小鼠斑秃模型的效果比较研究
Comparative study of effects of imiquimod and cyclophosphamide for inducing alopecia areata model in C57BL/6J mice
任贞妮 1许佳祺 2李娟 2孙荣军 2杨丽萍 2赵锋 2向柏 2张国强3
作者信息
- 1. 河北工程大学,河北 邯郸 056000
- 2. 河北医科大学研究生学院,石家庄 050017
- 3. 河北医科大学第一医院皮肤科,石家庄 050017
- 折叠
摘要
Abstract
Objective To establish and compare C57BL/6J mouse models of alopecia areata induced by imiquimod(IMQ)and cyclophosphamide(CTX).Methods A total of 104 C57BL/6J mice were divided into an IMQ group(5%0.05 g IMQ applied to the head/back,4 times a week,for 4 weeks),a CTX group(intraperitoneal injection of 3 mg/20 g CTX after hair removal),and a control group treated with Vaseline(0.05 g)or hair removal alone in the corresponding area.Body weight and hair changes were monitored continuously during the modeling process.Changes in skin hair follicles and epidermal thickness were observed by hematoxylin-eosin staining.Expression levels of interferon-γ(IFN-γ),interleukin-15(IL-15),and tumor necrosis factor-α(TNF-α)in mouse head and back skin lesions and serum were detected by enzyme-linked immunosorbent assay.Results The IMQ group cycle was 4 weeks,and a patchy bare area about 1 cm×1 cm developed on the head and back skin in week 4,accompanied by a small amount of scales.The CTX modeling cycle was 14 days,and the hair on the head and back lesions fell off 5 days after intraperitoneal CTX injection,and the skin appeared dark gray.Histopathology showed significant changes in hair follicles between the two model groups compared with the control group,with greater thickening of the epidermis in the IMQ group.Both IMQ and CTX significantly increased IFN-γ,IL-15,and TNF-α levels in skin and serum(P<0.0001),with a more pronounced increase in inflammatory factors in the IMQ group(P<0.0001,P<0.001,P<0.01).Conclusions Both IMQ and CTX can successfully induce alopecia areata on the head and back in C57BL/6J mice.Compared with IMQ,CTX modeling has the advantages of shorter modeling time,higher survival rate,and higher modeling rate,creating a relatively stable animal model of alopecia areata.关键词
斑秃/动物模型/环磷酰胺/咪喹莫特Key words
alopecia areata/animal models/cyclophosphamide/imiquimod分类
医药卫生引用本文复制引用
任贞妮,许佳祺,李娟,孙荣军,杨丽萍,赵锋,向柏,张国强..咪喹莫特与环磷酰胺诱导C57BL/6J小鼠斑秃模型的效果比较研究[J].中国比较医学杂志,2026,36(3):83-92,10.
