中国畜牧兽医2026，Vol.53Issue(3)：1473-1488,16.DOI:10.16431/j.cnki.1671-7236.2026.03.037
基于免疫信息学分析设计牛呼吸道合胞体病毒多表位疫苗
Design of a Multi-epitope Vaccine Against Bovine Respiratory Syncytial Virus Using Immunoinformatics Analysis
摘要
Abstract
[Objective]This study aimed to design a multi-epitope peptide vaccine targeting the F and G proteins of Bovine respiratory syncytial virus(BRSV)using immunoinformatics methods,with the aim of effectively activating T cell and B cell immune responses.[Method]Based on the antigen sequences of F and G proteins,B cell epitopes,cytotoxic T lymphocyte(CTL)epitopes,and helper T lymphocyte(HTL)epitopes were screened using bioinformatics analysis software,taking into account parameters such as antigenicity,allergenicity,and toxicity of each epitope.The selected B cell epitopes,CTL epitopes and HTL epitopes were connected using KK,AAY and GPGPG linkers,respectively.An adjuvant of β-defensin peptide 3 was added to the N-terminal of the constructed sequence,and a 6×His tag was added to the C-terminal.Protein-protein molecular docking was performed between the constructed vaccine protein sequence and Toll-like receptor 3(TLR3)and TLR4 using computational methods.The docking result with the largest cluster was selected for molecular dynamics(MD)simulation.Computer-based immune simulation,codon optimization and computer cloning were also conducted.[Result]A total of 5 B cell epitopes,7 CTL epitopes and 4 HTL epitopes were screened from the F and G antigens.The vaccine construct had good affinity and stability with the TLR3 and TLR4 models throughout the simulation period.Immune analysis of the vaccine demonstrated that it could significantly induce the production of various antibodies such as IgG and IgM,and also increase the levels of various cytokines.Through codon optimization,the GC content of the vaccine was 49.25%and the codon adaptation index(CAI)reached 1,indicating the potential for high expression in prokaryotic expression system.The vaccine was inserted into the Bam H Ⅰ and Xho Ⅰ restriction enzyme sites of the prokaryotic expression vector pET-28a(+),generating a complete cloning construct with a sequence length of 930 bp.[Conclusion]The multi-epitope vaccine designed in this study had the potential to induce strong T cell and B cell responses,and provided theoretical basis and data support for the development of BRSV multi-epitope vaccines.关键词
牛呼吸道合胞体病毒(BRSV)/F蛋白/G蛋白/B细胞表位/CTL表位/HTL表位Key words
Bovine respiratory syncytial virus(BRSV)/F protein/G protein/B cell epitope/CTL epitope/HTL epitope分类
农业科技引用本文复制引用
郭梓杰,王俊波,刘强,王璞,张刚,海梅梅,王元文,张思浓,李勇..基于免疫信息学分析设计牛呼吸道合胞体病毒多表位疫苗[J].中国畜牧兽医,2026,53(3):1473-1488,16.基金项目
肉牛重要传染病新型诊断与疫苗关键技术研究与应用(2024BBF02017) （2024BBF02017）
