中国畜牧兽医2026，Vol.53Issue(3)：1573-1584,12.DOI:10.16431/j.cnki.1671-7236.2026.03.045
1株鲫鱼致病性腐败希瓦氏菌的分离鉴定及中草药抑菌效果评价
Isolation,Identification of a Pathogenic Shewanella putrefaciens from Crucian Carp,and Evaluation of Antibacterial Effect of Chinese Herbal Medicine
摘要
Abstract
[Objective]This experiment was to explore the causes of crucian carp diseases in a fish farm in Jintan,Jiangsu province,so as to provide references for subsequent crucian carp breeding and the prevention and control of related diseases.[Method]Internal organ samples of diseased crucian carp were collected for bacterial isolation and purification.The isolated bacteria were preliminarily identified through morphological observation,electron microscopy observation and physiological and biochemical determination.The species relationship was determined by combining the 16S rDNA gene sequence and phylogenetic tree analysis.The pathogenicity of the isolated bacteria was detected by the regression infection test.Drug sensitivity test and antibacterial tests of Chinese herbal medicines were conducted by the disk diffusion method and the agar perforation method,respectively,to screen effective therapeutic drugs.[Result]The colonies of the isolated strain had smooth edges in the LB medium,presenting as milky white protrusions and were not hemolytic.Gram staining was negative and it appeared rod-shaped under transmission electron microscope.16S rDNA sequence analysis revealed that the isolated strain had a similarity of 99.17%to the Shewanella putrefaciens strain Hammer 95(NR_044866.1).On the phylogenetic tree,it naturally aggregated into the same branch with Shewanella putrefaciens ATCC 8071(NR_119141.1).It was determined that the isolated strain was Shewanella putrefaciens.The results of pathogenicity test showed that the same bacteria as the inoculated strain were re-isolated from the infected crucian carp.The results of drug sensitivity test and antibacterial test of Chinese herbal medicines showed that the isolated strain was sensitive to 28 kinds of antibacterial drugs such as ampicillin,minocycline,and amicacin,as well as 8 kinds of Chinese herbal medicines such as Galla chinensis and Prunus mume.Among them,Galla chinensis and Chebula retz had the best antibacterial effects,with both minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of 15.63 mg/mL.Moreover,Galla chinensis,Chebula retz and Syzygium aromaticum had a synergistic effect.[Conclusion]This study confirmed that Shewanella putrefaciens was the primary pathogen responsible for the infection and mortality of farmed crucian carp in Jintan,Jiangsu.Moreover,the isolated strain was sensitive to 28 kinds of antibacterial drugs such as ampicillin and minocycline,as well as 8 kinds of Chinese herbal medicines such as Galla chinensis and Chebula retz.This results provided a theoretical basis for the scientific prevention and control of Shewanella putrefaciens infection关键词
鲫鱼/腐败希瓦氏菌/药敏试验/致病性/中草药Key words
crucian carp/Shewanella putrefaciens/drug sensitivity test/pathogenicity/Chinese herbal medicine分类
农业科技引用本文复制引用
孙立,段蕊,姜虎成,薛晖,赵彦华..1株鲫鱼致病性腐败希瓦氏菌的分离鉴定及中草药抑菌效果评价[J].中国畜牧兽医,2026,53(3):1573-1584,12.基金项目
国家大宗淡水鱼产业技术体系(CARS-45) （CARS-45）
