中国瓜菜2026，Vol.39Issue(3)：15-26,12.DOI:10.16861/j.cnki.zggc.2025.0593
中国南瓜长短蔓性状的遗传规律及代谢组学分析
Genetic law and metabolomic analysis of long-and short-vine traits in Cucurbita moschata
摘要
Abstract
To elucidate the genetic and metabolic mechanisms underlying the differentiation of vine length traits in Chi-nese pumpkin(Cucurbita moschata),this study utilized homozygous short vine and long vine inbred lines to construct an F2 segregating population.Field phenotypic analysis revealed that the segregation ratio of short vine to long vine plants in F2 population fit a 3∶1(χ2=0.0981,P=0.754),suggesting that the short vine trait may be controlled by a single dominant nuclear gene.Morphologically,short vine materials were characterized by significantly shortened internodes,fewer nodes,increased stem diameter and distorted main vine,whereas long vine materials exhibited longer internodes and a greater number of nodes.Based on LC-MS/MS metabolomics analysis,a total of 1116 metabolites were identified at the vine stage.A total of 297 differential metabolites were obtained by preliminary screening,30 metabolites of which showed highly significant difference between the two vine materials.The up-regulated metabolites were mainly organic acids and their derivatives,along with organic oxygen compounds,while down-regulated metabolism were predominantly phenylpropanoids,polyketides,and organic oxygen compounds.KEGG enrichment analysis indicated that the differential accumulated metabolites were significantly enriched in metabolic pathways,including glycerophospholipid metabolism,ABC transporter,plant secondary metabolite biosynthesis and amino acid biosynthesis.Notably,glycerophospholipid me-tabolism and endogenous cannabinoid signal transduction may play a key role in the formation of different vine types.In conclusion,the short vine trait of C.moschata is controlled by a single dominant nuclear gene,and its formation is closely related to pathways such as glycerophospholipid metabolism and endogenous cannabinoid signal transduction.This trait manifests as a resource reallocation pattern centered on"growth-defense",characterized by enhanced cell wall thickening and secondary metabolism.These findings provide important targets and a theoretical basis for molecular breeding of pumpkin short vine varieties.关键词
中国南瓜/短蔓/蔓性性状/遗传规律/代谢组学Key words
Cucurbita moschata/Short vine/Vine trait/Genetic law/Metabolomics分类
农业科技引用本文复制引用
郭铭,程文婕,戴思慧,何长征,汤冰倩..中国南瓜长短蔓性状的遗传规律及代谢组学分析[J].中国瓜菜,2026,39(3):15-26,12.基金项目
国家西甜瓜产业技术体系(CARS-25) （CARS-25）
