中国中医药信息杂志2026，Vol.33Issue(3)：26-34,9.DOI:10.19879/j.cnki.1005-5304.202506262
基于网络药理学及实验验证的芪参益心颗粒干预慢性心力衰竭机制研究
Study on Intervention Mechanism of Qishen Yixin Granules on Chronic Heart Failure Based on Network Pharmacology and Experimental Validation
摘要
Abstract
Objective To investigate the mechanism of Qishen Yixin Granules on chronic heart failure(CHF)through network pharmacology and experimental validation.Methods Active components and targets of Qishen Yixin Granules were screened from TCMSP database.CHF-related targets were obtained from OMIM,GeneCards,TTD and DrugBank databases.The drug targets and disease targets were intersected,the protein-protein interaction network was constructed and the core targets were screened,and GO and KEGG pathways were enriched and analyzed.Molecular docking was performed to validate interactions between core components and targets.A CHF rat model was established by intraperitoneal injection of doxorubicin hydrochloride.Rats were randomly divided into control group,model group,Qili Qiangxin Capsule group,and Qishen Yixin Granules low-,medium-,high-dosage group.After 3 weeks of intervention,cardiac function(LVEF,LVFS)and serum NT-proBNP levels were detected.HE staining and Masson staining were used to observe the morphology of myocardial tissue.The expression of MMP2 was detected by immunohistochemistry,and the mRNA and protein expressions of TNF-α/p38 MAPK/CREB1 pathway of myocardial tissue were detected by RT-qPCR and Western blot.Results Network pharmacology identified key components including luteolin and quercetin in Qishen Yixin Granules.Enrichment analysis revealed MAPK,HIF-1 and NF-κB pathways as key pathways.Animal experiment results showed that Qishen Yixin Granules alleviated myocardial pathological changes,improved LVEF and LVFS,reduced serum content of NT-proBNP(P<0.01),attenuated myocardial fibrosis,and down-regulated mRNA and protein expressions of MMP2,TNF-α,p38 MAPK and CREB1(P<0.05,P<0.01).Conclusion Qishen Yixin Granules may inhibit inflammatory reaction and myocardial fibrosis by regulating the expression of MMP2 and TNF-α/p38 MAPK/CREB1 signaling pathway,so as to improve cardiac function.关键词
网络药理学/芪参益心颗粒/慢性心力衰竭/基质金属蛋白酶2/肿瘤坏死因子-α/p38丝裂原活化蛋白激酶/环磷腺苷效应元件结合蛋白1Key words
network pharmacology/Qishen Yixin Granules/chronic heart failure/MMP2/TNF-α/p38 MAPK/CREB1分类
医药卫生引用本文复制引用
马睿玲,伊琳,贾冠军,吕红英,王怡园,张玲娜,康万荣,徐晓东,卢玉俊,李应东..基于网络药理学及实验验证的芪参益心颗粒干预慢性心力衰竭机制研究[J].中国中医药信息杂志,2026,33(3):26-34,9.基金项目
国家自然科学基金(82160842) （82160842）
甘肃省联合科研基金一般项目(23JRRA1521) （23JRRA1521）
甘肃省教育厅高校教师创新基金项目(2026A-112) （2026A-112）
甘肃省自然科学基金(24JRRA1019) （24JRRA1019）
