中药材2025，Vol.48Issue(11)：2860-2867,8.DOI:10.13863/j.issn1001-4454.2025.11.031
芪竭消痞汤含药血清对MNNG诱导的人胃黏膜上皮细胞自噬及凋亡的影响
Effects of the Drug-Containing Serum of Qijie Xiaopi Decoction on Autophagy and Apoptosis of Human Gastric Mucosal Epithelial Cells Induced by MNNG
摘要
Abstract
Objective:To observe the effect of Qijie Xiaopi Decoction on the malignant transformation of gastric mucosal epithelial cells induced by MNNG,and to explore its mechanism of treating precancerous lesions of gastric cancer based on the PI3K/AKT/mTOR signaling pathway.Methods:The CCK-8 assay was used to determine the effective concentration of MNNG for inducing the malignant transformation of human gastric mucosal epithelial cells GES-1,and in vitro PLGC cell model(MC cells)was established.The blank control group,model group,the high,medium and low concentration groups of Qijie Xiaopi Decoction drug-containing serum,Ly294002(PI3K inhibitor)group were set up.The effect of Qijie Xiaopi Decoction on the proliferation of MC cells was detected by CCK-8 meth-od.The autophagy of MC cells was observed by MDC staining.The apoptosis rate of cells was determined by flow cytometry.The mRNA expressions of Bcl-2 and Bax were detected by qPCR.The protein expressions of PI3K,p-AKT,p-mTOR,AKT,p-mTOR,p62,LC3 and Beclin-1 were detected by Western blotting.Results:After interfering with GES-1 cells with MNNG at a concentration of 20 μmol/L for 48 hours,the cell inhibition rate reached about 50%,and at this concentration,GES-1 cells exhibited the biological characteristics of malignant transformation.At the same time points,compared with the GES-1 cell group,the viability of MC cells was significantly in-creased(P<0.01).Compared with the model group,the cell viability in the medium and high concentration groups of drug-containing serum of Qijie Xiaopi Decoction and the Ly294002 group was significantly decreased,while the number of autophagosomes,apoptosis rate,expression of Bax mRNA,and protein expressions of LC3 and Beclin-1 were significantly increased.The expressions of Bcl-2 mR-NA and p62,PI3K,p-AKT,p-mTOR proteins were significantly decreased(P<0.05 or P<0.01).Conclusion:Qijie Xiaopi Decoction can exert therapeutic effects on precancerous lesions of gastric cancer by inhibiting the proliferation of MC cells,promoting autophagy and apoptosis of cells.The related mechanism may be associated with the inhibition of the PI3K/AKT/mTOR signaling pathway.关键词
芪竭消痞汤/胃癌前病变/MC细胞/PI3K/AKT/mTOR/自噬/凋亡Key words
Qijie Xiaopi Decoction/Precancerous lesions of gastric cancer/MC cells/PI3K/AKT/mTOR/Autophagy/Apoptosis分类
医药卫生引用本文复制引用
单意,白敏,马洋,陈涵斐,史明珠,虎峻瑞..芪竭消痞汤含药血清对MNNG诱导的人胃黏膜上皮细胞自噬及凋亡的影响[J].中药材,2025,48(11):2860-2867,8.基金项目
宁夏回族自治区重点研发项目(2022BEG03150) （2022BEG03150）
宁夏医科大学科学研究资助项目研究计划(XZ2022006) （XZ2022006）
