中药材2025，Vol.48Issue(10)：2414-2420,7.DOI:10.13863/j.issn1001-4454.2025.10.002
三七叶际木霉的分离及其对三七圆斑病的防治研究
Isolation of Trichoderma from the Phyllosphere of Panax notoginseng and Application in the Control of Panax notoginseng Round Spot Disease
摘要
Abstract
Objective:To isolate the Trichoderma from the phyllosphere of Panax notoginseng and explore the control effect in round spot disease of Panax notoginseng.Methods:The fungi exhibiting typical characteristics of Trichoderma were preliminarily isolated using the coating method.The inhibitory effect of the isoloted strain on the mycelial growth of the causal agent of Panax notoginseng leaf spot disease was evaluated using the dual culture method.Furthermore,morphological and molecular biological techniques were em-ployed to identify the genus and species of the strain.Microscopic techniques were used to investigate the inhibitory effects of Trichoder-ma conidia on the germination and growth of conidia of the Panax notoginseng leaf spot pathogen.Finally,a pot experiment was conduc-ted to assess the efficacy of phyllospheric Trichoderma in the prevention and control of Panax notoginseng leaf spot disease.Results:A suspected strain of Trichoderma was isolated from the phyllosphere of Panax notoginseng.This strain exhibited an inhibition rate of 78.33%against Mycocentrospora acerina.Based on morphological characteristics and ITS sequence analysis,strain BY-10 was identified as Trichoderma atroviride.Potato dextrose peptone liquid medium was found to be the optimal medium for the activation and germination of Trichoderma atroviride conidia.The inhibition rate of Trichoderma atroviride conidia on the germination of conidia of the Panax notog-inseng round spot pathogen reached 49.32%.Pot experiments demonstrated that spraying Trichoderma atroviride conidia significantly re-duced the number of lesions caused by round spot disease on Panax notoginseng.Conclusion:A strain of Trichoderma atroviride is isola-ted from the phyllosphere of Panax notoginseng.The strain exhibits significant inhibitory effects on both the mycelial growth and conidial germination of the round spot pathogen,effectively decreasing the number of leaf lesions,it is a biocontrol strain with application poten-tial,the study provides a valuable reference for the field management of round spot disease in Panax notoginseng.关键词
三七/叶际微生物/深绿木霉/圆斑病Key words
Panax notoginseng(Burk.)F.H.Chen/Phyllosphere microorganisms/Trichoderma atroviride/Leaf spot disease分类
医药卫生引用本文复制引用
杨宽,黄显章,叶坤浩,李超,何霞红..三七叶际木霉的分离及其对三七圆斑病的防治研究[J].中药材,2025,48(10):2414-2420,7.基金项目
国家现代农业产业技术体系建设专项(CARS-21) （CARS-21）
南阳理工学院博士科研启动基金项目(NGBJ-2024-06) （NGBJ-2024-06）
