中国中医骨伤科杂志2026，Vol.34Issue(3)：1-6,6.DOI:10.20085/j.cnki.issn1005-0205.260301
苍柏丹防治骨科术后下肢深静脉血栓形成及炎症反应的实验研究
Experimental Study on the Efficacy of Cangbai Dan in Treating Lower Extremity Deep Venous Thrombosis and Inflammatory Response after Orthopedic Surgery
摘要
Abstract
Objective:To evaluate the effects of Cangbai Dan on deep vein thrombosis(DVT)formation and inflammation after major orthopedic surgery.Methods:Fifty rats were ran-domly assigned to five groups(n=10 each):blank,model,low-dose Cangbai Dan,high-dose Cangbai Dan,and positive drug group.Except for the blank group,all rats underwent DVT modeling.From day 5 post-surgery,rats received intra-gastric administration of Cangbai Dan(6.84 g/kg or 13.68 g/kg,twice daily),saline(2.5 mL,twice daily),or saline com-bined with low-molecular-weight heparin calcium(0.3 mL,subcutaneously,once daily)for five consecutive days.His-topathological changes in the great saphenous vein were evaluated by hematoxylin-eosin(HE)staining.Expression of IκBα and p65 proteins was assessed by immunohistochemistry and Western Blot.Serum IL-6,IL-8,and HMGB1 levels were measured by ELISA.Results:HE staining showed that Cangbai Dan and the positive drug protected the vascular endotheli-um,with superior effects in the high-dose group.ELISA demonstrated that IL-6,IL-8,and HMGB1 levels were lowest in the blank group and highest in the model group,with dose-dependent reductions in the Cangbai Dan groups.No significant difference was observed between the high-dose Cangbai Dan group and the positive drug group(P>0.05).Western Blot and immunohistochemistry showed consistent trends in IκBα and p65 expression.Conclusion:Cangbai Dan prevents post-operative lower limb DVT by inhibiting NF-κB pathway activation,downregulating NF-κB p65 and IκBα expression,and attenuating inflammatory responses.These findings support its therapeutic potential in the prevention of thrombosis after orthopedic surgery.关键词
苍柏丹/骨科大手术/下肢深静脉血栓/炎症/高迁移率族蛋白Key words
Cangbai Dan/major orthopedic surgery/lower extremity deep vein thrombosis/inflammation/high-mobility group histones分类
医药卫生引用本文复制引用
吴进,邱礼国,郭体康,唐勇,许晓彤,卢敏,邝高艳,谭开云..苍柏丹防治骨科术后下肢深静脉血栓形成及炎症反应的实验研究[J].中国中医骨伤科杂志,2026,34(3):1-6,6.基金项目
国家自然科学基金项目(82274543)湖南省自然科学基金项目(2023JJ30471)湖南省中医药管理局项目(B2024046,D2024042)湖南省教育厅优秀青年项目(24B0372)湖南省卫计委课题(202204074679)湖南省科技创新类湖湘青年英才项目(2024RC3203) （82274543）
