中药材2025，Vol.48Issue(10)：2453-2457,5.DOI:10.13863/j.issn1001-4454.2025.10.008
鉴别元宝草和贯叶金丝桃DNA条形码的筛选研究
Screening Study on DNA Barcoding of Hypericum sampsonii and Hypericum perforatum
摘要
Abstract
Objective:To screen and determine the DNA barcode sequences suitable for the identification of Hypericum sampsonii and Hypericum perforatum and seek feasible molecular identification method to help solve the morphological identification difficulties of Hypericum sampsonii and Hypericum perforatum.Methods:Twenty samples of Hypericum sampsonii and Hypericum perforatum were col-lected from various places.Genomic DNA was extracted.DNA barcoding universal primers ITS2,matK,psbA-trnH and rbcL were select-ed.Each sample was amplified according to the standard procedure.The genetic distances within and between species were calculated u-sing the Kimura-2-Parameter model(K2P),and the phylogenetic tree was constructed by the Neighbor-joining method.The ViennaRNA Web Services database was applied to predict the secondary structure of the candidate sequence.Results:The rbcL,psbA-trnH and ITS2 sequences were all amplified successfully and the sequencing success rate reached 100%.After comparison,it was found that the ITS2 sequence had the most variant sites and a higher interspecific K2P distance,which could distinguish between Hypericum sampsonii and Hypericum perforatum.In addition,there were significant differences in the secondary structures of ITS2,psbA-trnH and rbcL sequences between Hypericum sampsonii and Hypericum perforatum.Conclusion:ITS2 sequence is the preferred DNA barcode for the identification of Hypericum sampsonii and Hypericum perforatum,while psbA-trnH sequence can be used as an auxiliary identification barcode.The combination of two can effectively achieve the authenticity identification of the species of Hypericum sampsonii and Hypericum perfora-tum.关键词
元宝草/贯叶金丝桃/DNA条形码/分子鉴定Key words
Hypericum sampsonii Hance/Hypericum perforatum L./DNA barcoding/Molecular identification分类
医药卫生引用本文复制引用
李金霖,唐振航,李丹蕾,马艺嘉,张红伟,梁爽..鉴别元宝草和贯叶金丝桃DNA条形码的筛选研究[J].中药材,2025,48(10):2453-2457,5.基金项目
广西科技基地和人才专项(桂科AD21238031) （桂科AD21238031）
壮瑶药协同创新中心(桂教科研[2013]20号) （桂教科研[2013]20号）
广西重点研发计划项目(桂科AB21196016) （桂科AB21196016）
广西壮瑶药重点实验室(桂科基字[2014]32号) （桂科基字[2014]32号）
