中国中医骨伤科杂志2026，Vol.34Issue(3)：36-42,7.DOI:10.20085/j.cnki.issn1005-0205.260306
木犀草素调控STAT1/Caspase-3通路对激素性股骨头坏死大鼠组织形态的影响
Effect of Luteolin on the Tissue Morphology of Steroid-Induced Osteonecrosis of the Femoral Head in Rats by Regulating STAT1/Caspase-3 Pathway
摘要
Abstract
Objective:To discuss the effect of luteolin on the tissue morphology of steroid-induced osteonecrosis of femoral head(SONFH)rats by regulating signal transducer and activator of transcription 1(STAT1)/Cysteinyl aspartate specific proteinase 3(Caspase-3)pathway.Methods:Rats were randomly grouped into the control group,model group,luteolin low-dose group,luteolin high-dose group,and luteolin high-dose+Fludarabine(STAT1 activator)group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),bone alkaline phosphatase(BALP),procollagen I N-terminal propeptide(PINP),tartrate-resistant acid phos-phatase(TRAP),and β-collagen degradation product(β-CTX)in rat serum.The apoptosis of femoral structure and bone tissue cells was detected.Hematoxylin-Eosin(HE)staining was performed to detect the morphology of femoral tissue.Western Blot was performed to detect the Runt-relat-ed transcription factor 2(Runx2),bone morphogenetic protein-2(BMP-2),Cleaved cysteine-aspartic acid protease-3(Cleaved Caspase-3),and STAT1/Caspase-3 pathway proteins.Results:For the control group,the femoral head structure of rats in the model group was markedly destroyed,and the femoral head tissue was prominently damaged.The serum IL-1β,IL-6,TNF-α,TRAP and β-CTX,trabecular separation(Tb.Sp),empty bone resorption rate,bone tissue cell apoptosis rate,Cleaved Caspase-3,p-STAT1/STAT1 and Caspase-3 proteins were increased,while the serum BALP and PINP,bone volume fraction(BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th),and the bone tissue Runx2 and BMP-2 proteins were decreased(P<0.05).For the model group,the degree of structural damage to the femoral head of rats in the luteolin low-dose,luteolin high-dose groups was reduced,and the morphology of the femoral head tissue was prominently improved.The serum IL-1β,IL-6,TNF-α,TRAP and β-CTX,Tb.Sp,empty bone resorption rate,bone tissue cell apoptosis rate,Cleaved Caspase-3,p-STAT1/STAT1 and Caspase-3 proteins were decreased,while the serum BALP and PINP,BV/TV,Tb.N,Tb.Th,and the bone tissue Runx2 and BMP-2 proteins were increased(P<0.05).Fludara-bine could reduce the improvement effect of luteolin on bone tissue morphology in SONFH rats(P<0.05).Conclusion:Luteolin may alleviate femoral head tissue damage in SONFH rats by inhibiting STAT1/Caspase3 pathway.关键词
木犀草素/STAT1/Caspase-3通路/激素性股骨头坏死/大鼠/组织形态Key words
luteolin/STAT1/Caspase-3 pathway/steroid-induced osteonecrosis of femoral head/rats/morphology分类
医药卫生引用本文复制引用
夏静,唐光平,李小平,靳艳..木犀草素调控STAT1/Caspase-3通路对激素性股骨头坏死大鼠组织形态的影响[J].中国中医骨伤科杂志,2026,34(3):36-42,7.基金项目
湖北省中医药管理局中医药科研项目(ZY2023Z011) （ZY2023Z011）
