中国中医骨伤科杂志2026，Vol.34Issue(3)：51-56,6.DOI:10.20085/j.cnki.issn1005-0205.260308
橘红素对白细胞介素-1β诱导的软骨细胞损伤的影响
The Effect of Tangeretin on Interleukin-1β-Induced Chondrocyte Damage by Regulating the AMPK/SIRT1/NF-κB Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of tangeretin on interleukin-1β(IL-1β)-induced chondrocyte injury and ana-lyze its mechanistic association with the AMP-activated protein kinase(AMPK)/silent information regulator 2 homolog 1(SIRT1)/nuclear factor κB(NF-κB)signaling pathway.Methods:Chondrocytes were cultured and divided into control group,interleukin-1β group,low-dose,medium-dose,and high-dose tangeretin groups,and high-dose tangeretin plus AMPK inhibitor Compound C group.Cloning formation assays,flow cytometry,DCFH-DA fluorescent probes,and West-ern Blot were used to detect cell proliferation,apoptosis,ROS levels,Collagen Ⅱ,MMP-3,MMP-13,and the expression of proteins related to the AMPK/SIRT1/NF-κB pathway.Results:The interleukin-1β group showed reduced clone formation number,SOD levels,Collagen Ⅱ,SIRT1 expression,p-AMPK/AMPK ratio,as well as increased apoptosis rate,IFN-γ,IL-6,TNF-α,MDA levels,fluorescence intensity,MMP-3,MMP-13 expression,and Ac-NF-κB p65/NF-κB p65 ratio compared to the control group(P<0.05).The low-dose,medium-dose,and high-dose tangeretin groups showed increased clone for-mation number,SOD levels,Collagen Ⅱ,SIRT1 expression,p-AMPK/AMPK ratio,as well as reduced apoptosis rate,IFN-γ,IL-6,TNF-α,MDA levels,fluorescence intensity,MMP-3,MMP-13 expression,and Ac-NF-κB p65/NF-κB p65 ratio compared with the interleukin-1β group(P<0.05).The high-dose tangeretin+Compound C group showed re-duced clone formation number,SOD level,Collagen Ⅱ,SIRT1 expression,p-AMPK/AMPK ratio,increased apoptosis rate,IFN-γ,IL-6,TNF-α,MDA levels,fluorescence intensity,MMP-3,MMP-13 expression,and Ac-NF-κB p65/NF-κB p65 ratio compared to the high dose tangeretin group(P<0.05).Conclusion:The improvement effect of tangeretin on IL-1β-induced chondrocyte damage may be achieved by activating the AMPK/SIRT1/NF-κB signaling pathway.关键词
橘红素/AMPK/SIRT1/NF-κB通路/白细胞介素-1β/软骨细胞/损伤Key words
tangeretin/AMPK/SIRT1/NF-κB pathway/interleukin-1β/chondrocytes/damage分类
医药卫生引用本文复制引用
王亮,许治国,杨轶..橘红素对白细胞介素-1β诱导的软骨细胞损伤的影响[J].中国中医骨伤科杂志,2026,34(3):51-56,6.基金项目
河北省中医药管理局科研计划项目(2024425) （2024425）
