北方园艺Issue(5)：1-9,9.DOI:10.11937/bfyy.20252697
菜豆复合诱变突变体库构建及基因组分析
Construction of a Composite Mutagenized Snap Bean Mutant Library and Genomic Analysis
摘要
Abstract
Taking seeds of dwarf edible-podded snap bean(Phaseolus vulgaris)A18 as experimental material,a combined mutagenesis method involving nitrogen ion beam(4 × 1016 ions·cm-2)and 60Co-γ ray(80 Gy)irradiation was applied to investigate the effects of composite mutagenesis on phenotypic variation in snap bean and the genomic characteristics of the mutants,in order to create foundational materials for genetic breeding and functional genomics research in snap bean and provide reference for composite mutagenesis research in related crops.The results showed that a total of 374 mutant plants belonging to five phenotypic categories-leaf,flower/fertility,stem/plant architecture,seed,and growth period were screened in the M2 generation,with a mutation frequency of 6.34％.Nine stably inherited mutant lines were selected,advanced to the M3 generation,and subjected to whole-genome resequencing analysis.Significant differences were observed among mutants in nucleotide diversity(Pi:0.184-0.195)and genetic differentiation coefficient(Fst:0.111-0.172).The genetic similarity coefficients between mutants and the wild type ranged from 0.83 to 0.87.关键词
食荚菜豆(Phaseolus vulgaris L.)/复合诱变/突变体库/全基因组重测序Key words
Phaseolus vulgaris L./combined mutagenesis/mutant library/whole-genome resequencing分类
农业科技引用本文复制引用
斯琴图雅,张子呈,张丹丹,汤明威,孙培琳,高娃..菜豆复合诱变突变体库构建及基因组分析[J].北方园艺,2026,(5):1-9,9.基金项目
黑龙江省科学院青年创新基金资助项目(QNCX2024YZN02). （QNCX2024YZN02）
