北方园艺Issue(5)：21-29,9.DOI:10.11937/bfyy.20252223
黑果枸杞LrWRKY44的克隆、生物信息学及差异表达分析
Cloning,Bioinformatics and Expression Analysis of LrWRKY44 in Lycium ruthenicum Murr.
摘要
Abstract
Taking Lycium ruthenicum fruit,root,stem,leaf,and flower as the experimental materials,the structure of LrWRKY44 was analyzed using bioinformatics tools,and its expression patterns in different tissues and at different developmental stages were determined by RT-qPCR,in order to preliminarily explore its biological function in the growth and development of L.ruthenicum.The results showed that LrWRKY44 was expressed in all parts of L.ruthenicum,including roots,stems,leaves,flowers,and fruits,with the highest expression level found in fruits.As the fruit matures,the expression of LrWRKY44 gradually increases,peaking at maturity,which aligns with the accumulation trend of anthocyanin content.The results indicated that LrWRKY44 might be closely related to the biosynthesis and accumulation of anthocyanins in L.ruthenium and played an important regulatory role in the formation of fruit color.关键词
黑果枸杞/LrWRKY44/生物信息学分析/表达特性Key words
Lycium ruthenicum Murr./LrWRKY44/bioinformatics analysis/expression analysis分类
农业科技引用本文复制引用
郭瑾,吴永成,覃彩芳,刘慧,王静..黑果枸杞LrWRKY44的克隆、生物信息学及差异表达分析[J].北方园艺,2026,(5):21-29,9.基金项目
北方民族大学研究生创新资助项目(YCX24405) （YCX24405）
国家自然科学基金资助项目(32160392) （32160392）
宁夏回族自治区重点研发计划资助项目(2021BEF02002). （2021BEF02002）
