波谱学杂志2026，Vol.43Issue(1)：37-45,9.DOI:10.11938/cjmr20253164
以吉拉德试剂T作为NMR探针研究人源细胞色素c氧化修饰的影响因素
Investigating the Factors Influencing Oxidative Modification of Human Cytochrome c Using Girard's Reagent T as an NMR Probe
摘要
Abstract
Oxidative modification of cytochrome c(Cyt c)may influence the local conformation of protein,yet the mechanism by which structural alterations of Cyt c affect its degree of oxidative modification remains unclear.In this study,Girard's reagent T(GRT)was employed as a nuclear magnetic resonance(NMR)probe to investigate the oxidative modification levels of human Cyt c under varying environmental conditions.Experimental results demonstrated that protecting lysine residues through reductive methylation effectively reduced protein oxidation.Partial unfolding of Cyt c was found to enhance its oxidative modification,while binding Cyt c with cardiolipin significantly increased the extent of oxidation.Additionally,other factors such as protein aggregation exhibited inhibitory effects on oxidative modification.关键词
细胞色素c/氧化修饰/吉拉德试剂T/甲基化/核磁共振Key words
cytochrome c/oxidative modification/Girard's reagent T/methylation/NMR分类
数理科学引用本文复制引用
张广庆,占建华,肖雄,朱勤俊,蒋滨,刘买利,张许..以吉拉德试剂T作为NMR探针研究人源细胞色素c氧化修饰的影响因素[J].波谱学杂志,2026,43(1):37-45,9.基金项目
湖北省自然科学基金创新群体项目(2023AFA041). （2023AFA041）
