苏州工学院学报2026，Vol.40Issue(2)：31-38,112,9.
荷叶多糖NFP-0.8-b的纯化、磷酸化修饰及其生物活性
Purification,Phosphorylation Modification and Biological Activity of a Novel Polysaccharide NFP-0.8-b from Nelumbinis Folium
摘要
Abstract
Polysaccharides from Nelumbinis Folium(NFP)were extracted with deionized water as the solvent.A novel polysaccharide NFP-0.8-b was purified by DEAE-Sepharose Fast Flow anion exchange column and Sephadex G-100 gel chromatography column.On this basis,the optimal phosphorylation conditions for NFP-0.8-b were determined.The antioxidant activity of phosphorylated NFP-0.8-b(Ph-NFP-0.8-b)and its inhibitory effect on advanced glycation end products during cookie production were investigated.The results show that the optimal phosphorylation process of NFP-0.8-b is as follows:phosphorylation reagent ratio(sodium tripolyphosphate︰sodium trimetaphosphate)5︰2,reaction temperature 75℃,pH 9.0,and reaction time 3.5 h.Under these conditions,the phosphate content reaches 8.39%.Compared with NFP-0.8-b,the scavenging effect of Ph-NFP-0.8-b on DPPH free radicals and hydroxyl free radicals are further enhanced.When the polysaccharide content is greater than 5%,the inhibitory effect of Ph-NFP-0.8-b on advanced glycation end products during cookie production is significantly stronger than that of NFP-0.8-b(P＜0.05),suggesting that Ph-NFP-0.8-b could be used as an antioxidant and inhibitor of advanced glycation end products in the industrial production of cookies.关键词
荷叶多糖/磷酸化/晚期糖基化末端产物/曲奇/抗氧化Key words
polysaccharide from Nelumbinis Folium/phosphorylation/advanced glycation end products/cookie/antioxidant分类
轻工纺织引用本文复制引用
周南南,朱东兴,彭英云,陈义勇,荣可瑜,柏雪,张宁,陆庆萍,潘诗雯,余永平,陈福林,范婧妍..荷叶多糖NFP-0.8-b的纯化、磷酸化修饰及其生物活性[J].苏州工学院学报,2026,40(2):31-38,112,9.基金项目
2024年度江苏省大学生创新创业训练计划项目"荷叶多糖磷酸化修饰及其抗氧化活性"(202410333079Y) （202410333079Y）
2023年度常熟市关键核心技术攻关(社会发展)项目"焙烤类食品生产过程中危害物AGEs控制关键技术及应用研究"(CS202309) （社会发展）
