淡水渔业2026，Vol.56Issue(2)：68-78,11.
一株黄颡鱼源鮰爱德华菌的药敏特性及全基因组测序分析
Antimicrobial susceptibility and whole-genome sequencing and analysis of an Edwardsiella ictaluri strain YCH from yellow catfish
摘要
Abstract
Edwardsiella ictaluri is an important pathogen in aquaculture.In recent years,its increasing antibiotic resistance and cross-species transmission have raised serious concerns.Genomic studies of strains from different hosts are essential for exploring their pathogenic mechanisms,epidemiology,genetic diversity,and adaptive e-volution.To elucidate the antimicrobial resistance,genomic characteristics,and evolutionary relationships of an E.ictaluri strain YCH from yellow catfish,antimicrobial susceptibility testing and whole-genome sequencing and assembly were performed.Functional annotation was performed using multiple databases,and comparative and pan-genome analyses were conducted with nine other Edwardsiella strains.The results showed that the YCH ge-nome is 3.79 Mb in size,with a GC content of 57.42%,and comprises 3 439 coding sequences,25 rRNAs,and 99 tRNAs.A total of 34 genomic islands and 6 prophages were identified,while no CRISPR-Cas loci were detected.Antimicrobial susceptibility testing and resistance gene prediction indicated that the YCH strain might employ efflux pumps and target modification mechanisms to exhibit multidrug resistance to polymyxins,erythro-mycin,tetracyclines,and fluoroquinolones.Functional annotation revealed a large number of genes involved in metabolic pathways,with 3 564,3 078,and 3 384 genes assigned to GO,COG,and KEGG databases,respectively.Pan genomic analysis showed that YCH shares 2 294 conserved core genes with nine other Ed-wardsiella strains,while the number of strain-specific genes varied significantly(29～547).Phylogenetic analysis further revealed that YCH clusters closely with E.ictaluri Ei-VN5 in the same evolutionary branch.This study represents the first report of whole-genome sequencing and functional annotation of E.ictaluri strain YCH from yellow catfish,systematically revealing its genomic characteristics,functional distribution,antibiotic resistance,and evolutionary relationships.These findings provide an important insights into the pathogenicity and resistance mechanisms of E.ictaluri and offer valuable references for developing prevention and control strategies.关键词
鮰爱德华菌(Edwardsiella ictaluri)/全基因组测序/比较基因组/耐药性/毒力因子Key words
Edwardsiella ictaluri/whole-genome sequencing/comparative genomics/antibiotic resistance/viru-lence factors分类
农业科技引用本文复制引用
孔乐,郭锦志,龚建森,魏文志,刘晓丹,付立霞..一株黄颡鱼源鮰爱德华菌的药敏特性及全基因组测序分析[J].淡水渔业,2026,56(2):68-78,11.基金项目
江苏省农业科技自主创新资金项目(CX(24)3066) （CX(24）
农业农村部渔用药物创制重点实验室开放课题(201801) （201801）
