动物医学进展2026，Vol.47Issue(3)：1-8,8.
基于T3SS的沙门氏菌抑制剂筛选系统构建与多酚类化合物活性评价
Construction of a Screening System for Salmonella Inhibitors Based on T3SS and Activity Evaluation of Polyphenolic Compounds
摘要
Abstract
The type Ⅲ secretion system(T3SS)is a crucial virulence factor of Salmonella.Specifie inhibi-tors targeting T3SS is considered an effective method for combating Salmonella infections.This study uti-lized Red homologous recombination technology to construct an invA gene deletion strain of Salmonella Ty phi murium(SL1344 ΔinvA).A seamless cloning method was employed to construct the pWSK29-sopE2-luc reporter plasmid.HeLa cell infection model of Salmonella typhimurium was established at a multiplicity of infection(MOI)of 100 to set up a screening system for T3SS inhibitors.Polyphenolic com-pounds were screened to obtain natural product inhibitors of T3SS.The results show that the SL1344ΔinvA strain,as well as the SL1344(pWSK29-sopE2-luc)and SL1344ΔinvA(pWSK29-sopE2-luc)reporter strains,were successfully obtained.Through the T3SS inhibitor screening system,this study suc-cessfully identified effective T3SS inhibitors,including ethyl gallate,propyl gallate,caffeic acid,p-coumaric acid and tannic acid.This study successfully established a screening system for T3SS inhibitors,providing a platform for drug screening targeting virulence factors of Salmonella,and evaluated polyphenolic com-pounds to identify effective T3SS inhibitors.关键词
鼠伤寒沙门氏菌/Ⅲ型分泌系统(T3SS)/毒力抑制/Red同源重组/多酚类化合物Key words
Salmonella typhimurium/Type Ⅲ secretion system(T3SS)/Virulence inhibition/Red homolo-gous recombination/Polyphenols分类
农业科技引用本文复制引用
茹梦珂,刘盼盼,李穗湘,杨珍,郝宝成,王胜义,胡庭俊,崔东安..基于T3SS的沙门氏菌抑制剂筛选系统构建与多酚类化合物活性评价[J].动物医学进展,2026,47(3):1-8,8.基金项目
国家重点研发计划项目(2023YFD1800802) （2023YFD1800802）
