东南园艺2026，Vol.14Issue(1)：35-43,9.DOI:10.20023/j.cnki.2095-5774.2026.01.004
基于RNA-seq分析粉葛与野葛淀粉积累相关基因的差异表达特征
Comparative transcriptome analysis reveals differential expression of starch accumulation-related genes in Pueraria montana var.thomsonii and Pueraria montana var.lobata
摘要
Abstract
[Objective]This study aimed to investigate the key factors underlying the differential starch accumulation between Pueraria montana var.thomsonii(P.thomsonii)and Pueraria montana var.lobata(P.lobata),to provide a theoretical basis for the molecular breeding of high-starch kudzu varieties.[Method]Six medicinal and edible kudzu resources were collected.The morphological and starch granule characteristics of one-year-old roots were analyzed.Differential transcriptome analysis was performed to identify differentially expressed genes(DEGs),and correlation analysis was conducted to pinpoint genes associated with starch accumulation.[Result]The P.thomsonii accession G1 exhibited the highest starch content,which was twice that of P.lobata,and demonstrated more rapid development and expansion of secondary structural tissue cells.Transcriptome analysis revealed 4718 DEGs,predominantly enriched in functional categories including biological regulation,cellular membrane structures,extracellular regions,plasmodesmata,transcription factors,and signal transduction.Furthermore,137 and 231 genes were significantly positively and negatively correlated with starch accumulation,respectively.Among these,nine genes involved in starch and sugar metabolism and twelve transcription factors were identified as potential key regulators of starch accumulation specificity.[Conclusion]This study establishes a comparative transcriptome database and identifies critical gene set influencing starch accumulation,thereby providing a solid theoretical foundation for the transcriptional regulation and molecular breeding of P.thomsonii.关键词
粉葛/野葛/淀粉积累特异性/转录组分析/差异表达基因Key words
Pueraria montana var.thomsonii/Pueraria montana var.lobata/starch accumulation specificity/transcriptome analysis/differentially expressed genes分类
农业科技引用本文复制引用
郑天润,赵晓,谭均,吕卉,莫让瑜,陈大霞..基于RNA-seq分析粉葛与野葛淀粉积累相关基因的差异表达特征[J].东南园艺,2026,14(1):35-43,9.基金项目
重庆市技术创新与应用发展专项重点项目(cstc2021jscx-gksbX0015) （cstc2021jscx-gksbX0015）
