东南园艺2026，Vol.14Issue(1)：67-72,6.DOI:10.20023/j.cnki.2095-5774.2026.01.008
基于环介导等温扩增的石蒜绵粉蚧可视化快速检测
Visual and rapid detection of Phenacoccus solani based on loop-mediated isothermal amplification
摘要
Abstract
[Objective]Phenacoccus solani is a global pest of fruits and flowers.It is necessary to developed a visual and rapid detection method for the control of this mealybug.[Method]Based on the 28S rDNA sequence of P.solani,the specific primers of loop-mediated isothermal amplification(LAMP)were designed to establish the corresponding visual detection method,and the specificity,sensitivity and stability of LAMP detection were verified.[Result]The LAMP detection at 64℃for 60 min effectively detected the DNA of P.solani,it had a detection limit of 100 fg/µL and the detection ratio of 95.83%～100.00%with the positive blue reaction products.And the LAMP detection could not amplify the DNA of other mealybug species with the negative purple reaction products.[Conclusion]The LAMP visual and rapid detection is specific,sensitive and reliable for identification of P.solani on-site.关键词
石蒜绵粉蚧/环介导等温扩增/可视化快速检测/现场识别Key words
Phenacoccus solani/loop-mediated isothermal amplification/visual and rapid detection/on-site identification分类
农业科技引用本文复制引用
张杰,蓝炎阳,姚锦爱,余德亿,黄鹏..基于环介导等温扩增的石蒜绵粉蚧可视化快速检测[J].东南园艺,2026,14(1):67-72,6.基金项目
福建省自然科学基金项目(2024J01324) （2024J01324）
福建省属公益类科研院所基本科研专项(2024R1023004) （2024R1023004）
