广东农业科学2026，Vol.53Issue(1)：35-47,13.DOI:10.16768/j.issn.1004-874X.2026.01.004
72份水稻种质材料中抗白叶枯病分子标记检测分析
Molecular Marker Detection and Analysis of Resistance to Bacterial Blight in 72 Rice Germplasms
摘要
Abstract
[Objective]In order to address the challenges posed by bacterial blight to the safety of rice production and to clarify the status of bacterial blight resistance genes in 72 rice germplasm materials.[Method]The total DNA of 72 rice germplasm materials from the sea rice germplasm repository of Guangdong Ocean University was extracted.Use specific molecular marker techniques to conduct PCR and agarose gel electrophoresis for detection and analysis of the 8 known bacterial blight resistance genes(Xa1,Xa5,Xa7,Xa13,Xa21,Xa23,Xa26,Xa27).And the electrophoresis data were subjected to cluster analysis using the software NTsyspc 2.10e.[Result]The 72 rice germplasm materials were all found to possess resistance genes against bacterial blight,and each of the materials carried at least 2 such resistance genes simultaneously.Among them,the germplasm materials R1141 and D2 simultaneously carry five resistance genes,namely Xa1,Xa5,Xa7,Xa23,and Xa26.There were 53 samples carrying all three resistance genes simultaneously,accounting for 73.61%.A total of 51 germplasm materials carried the same resistance gene combination,namely Xa1,Xa5,and Xa26.Xa1,Xa26,and Xa5 were detected with the highest frequencies,which were 98.61%,93.06%,and 91.67%respectively.Cluster analysis divided 72 rice germplasm materials into 3 major categories:The I category included 64 materials,all of which carried both the Xa1 and Xa26 resistance genes simultaneously;the II category included 5 materials,all of which carried both the Xa1 and Xa5 resistance genes simultaneously;the III category included 3 materials,all of which carried the Xa1,Xa7,Xa23,and Xa26 resistance genes simultaneously.[Conclusion]The homogenization of bacterial blight resistance genes in rice germplasm materials is severe,and the polymorphism is relatively low.The proportion of materials carrying Xa1,Xa5 and Xa26 simultaneously is as high as 71%.However,there are only two germplasm materials carrying five resistance genes simultaneously,which can be used as source parents of disease-resistant genes and their innovative utilization should be strengthened.At the same time,efforts should be made to strengthen the protection of wild rice resources and local rice varieties resistant to bacterial blight,to preserve the diversity of rice germplasm,to discover more new genes for resistance to bacterial blight,and to widely utilize them in hybrid breeding to broaden the genetic background of rice resistance to bacterial blight,and to select more new materials that combine different combinations of disease-resistant genes.关键词
水稻/白叶枯病/抗性基因/分子标记/抗病育种Key words
Oryza sativa L./bacterial blight/resistance genes/molecular markers/disease resistance breeding分类
农业科技引用本文复制引用
何颖,许泽良,张弘,周鸿凯,莫俊杰,杨善..72份水稻种质材料中抗白叶枯病分子标记检测分析[J].广东农业科学,2026,53(1):35-47,13.基金项目
广东省重点领域研发计划项目(2020B020219004) （2020B020219004）
广东省乡村振兴战略专项种业振兴行动项目(2023-NBH-00-025) （2023-NBH-00-025）
广东省科技创新战略专项资金项目(2023A01039) （2023A01039）
