广东农业科学2026，Vol.53Issue(1)：67-81,15.DOI:10.16768/j.issn.1004-874X.2026.01.007
香蕉枯萎病菌检测技术研究进展
Research Progress on Detection Technologies for Fusarium Wilt of Banana
摘要
Abstract
Fusarium wilt of banana is a devastating soil-borne disease caused by Fusarium oxysporum f.sp.cubense(Foc).Due to the lack of effective management strategies,the disease is difficult to eradicate once occurred,and the pathogen can persist in soil for several decades,posing a severe threat to the global banana industry.The development of rapid and accurate early diagnostic techniques is therefore crucial for timely disease control and for ensuring sustainable banana production.Recently,traditional diagnostic method,PCR molecular detection technology,isothermal amplification technology,biosensor technology as well as spectroscopy and remote sensing tehndogy are used in Foc detection.Various detection methods are compared and analyzed in terms of detection targets,detection principles,advantages and disadvantages,and applicable scenarios.Traditional diagnostic method can meet basic identification needs but are time-consuming,lack accuracy,and are highly dependent on professional personnel.PCR molecular detection technology offer high sensitivity,rapid detection,and high accuracy,yet they rely on known specific sequence information,require specialized equipment,and are generally limited to laboratory-based qualitative or quantitative analysis.Isothermal amplification technology reduce dependence on sophisticated instruments and enable on-site rapid detection,however,they are prone to producing non-specific amplification or false positives due to aerosol pollution.Biosensor technology feature simple operation,rapid detection and miniaturization of equipment,but their accuracy remains limited,and the sensitivity is highly dependent on the specificity of detection targets.Spectroscopic and remote sensing technology enable rapid,intuitive,and non-destructive detection,but they often suffer from insufficient specificity and are sensitive to environmental interference.Based on recent advances,future research directions for Foc detection are prospected,including the development of new highly specific detection targets,optimization of on-site rapid detection systems,and the integrated application of multiple technologies.These efforts are expected to facilitate the establishment and application of protable,accurate,rapid,easy-to-operate and low-cost detection techniques for Foc,thereby supporting the green and efficient management of Fusarium wilt of banana and promoting the high-quality development of the banana industry.关键词
香蕉枯萎病/尖孢镰刀菌古巴专化型/检测技术/检测靶标Key words
Fusarium wilt of banana/Fusarium oxysporum f.sp.cubense/detection techniques/target detection分类
农业科技引用本文复制引用
陈文娟,毕方铖,李春雨,项丹丹,刘思文,黄火青,杨晓芳,杨转英..香蕉枯萎病菌检测技术研究进展[J].广东农业科学,2026,53(1):67-81,15.基金项目
国家重点研发计划项目(2024YFD1401104) （2024YFD1401104）
广东省农业科学院现代种业创新能力提升工程(2025ZYTS0405) （2025ZYTS0405）
广东省一流专业建设点项目(010306052103) （010306052103）
