南方医科大学学报2026，Vol.46Issue(3)：497-504,8.DOI:10.12122/j.issn.1673-4254.2026.03.03
盐酸甲氟喹促进DNA修复减轻辐射诱导的肺上皮细胞损伤
Mefloquine HCl promotes DNA repair and alleviates radiation-induced lung epithelial cell injury
摘要
Abstract
Objective To investigate the protective effect of mefloquine HCl(MQ)against X-ray irradiation-induced DNA damage in lung epithelial cells.Methods Human lung epithelial cells(BEAS-2B)were divided into blank control group,irradiation group,and irradiation+MQ treatment group.The effects of MQ on cell proliferation and radiosensitivity after X-ray irradiation were assessed using CCK-8 assay,EdU-488 assay,and colony formation assay.Apoptosis and cell cycle distribution of BEAS-2B cells with different treatments were detected by flow cytometry.The effect of MQ in promoting DNA double-strand break(DSB)repair was observed using immunofluorescence staining and comet assay,and the molecule ar mechanism was explored using Western blotting,qPCR,and luciferase reporter assays.Results MQ at 0-10 μmol/L did not significantly affect BEAS-2B cell viability.Compared to the irradiated cells,treatment with 0-10 μmol/L MQ enhanced the cell viability,and the effect was the most conspicuous at 10 μmol/L.MQ treatment obviously promoted proliferation and increased clonogenic survival rate of irradiated BEAS-2B cells while reducing their radiosensitivity,and significantly lowered cell apoptosis rate following the irradiation.Cell cycle analysis revealed that MQ alleviated G2/M phase arrest induced by irradiation,and comet assay and immunofluorescence staining showed reduced comet tail moment and γH2AX foci formation in irradiation+MQ group.Western blotting and qPCR demonstrated that MQ treatment for 48 h significantly increased CtIP promoter activity and upregulated CtIP expressions at both the mRNA and protein levels.Conclusion MQ promotes DSB repair in BEAS-2B cells by upregulating CtIP expression and may thus alleviate radiation-induced lung epithelial cell injury.关键词
盐酸甲氟喹/放射性肺损伤/DNA损伤/DNA双链断裂修复Key words
mefloquine HCl/radiation-induced lung injury/DNA damage/DNA double-strand break repair引用本文复制引用
张燕,温庆秋,黄海波,周美娟,王建宇..盐酸甲氟喹促进DNA修复减轻辐射诱导的肺上皮细胞损伤[J].南方医科大学学报,2026,46(3):497-504,8.基金项目
国家自然科学基金(81903256) （81903256）
广州市市校(院)企联合资助专题(广州市职业环境与健康重点实验室)(2024A03J0434)Supported by National Natural Science Foundation of China(81903256). （院）
