摘要
Abstract
Objective To investigate the expression levels of miR-423-5p and miR-328-3p in pregnant women with gestational diabetes mellitus(GDM)and their correlation with insulin resistance.Methods From April 2022 to April 2024,240 pregnant women who underwent prenatal examination and GDM screening in the Department of Obstetrics and Gynecology of Xuzhou Central Hospital were enrolled in this study.They were divided into the GDM group(study group,n=120)and the normal pregnancy group(control group,n=120).The expression levels of miR-423-5p and miR-328-3p were detected by quantitative real-time PCR(qRT-PCR).The general clinical data and insulin resistance-related indicators were compared between the two groups.Pearson correlation analysis was used to analyze the correlation between the levels of miR-423-5p and miR-328-3p in GDM pregnant women,as well as the correlations between these two microRNAs and insulin resistance indicators.Multivariate logistic regression analysis was applied to identify the factors influencing the occurrence of GDM.Results The levels of miR-423-5p and miR-328-3p in the study group were significantly higher than those in the control group,with statistically significant differences(t-values=7.267 and 7.556,respectively,P<0.05).In terms of biochemical indicators,the study group had higher levels of TC,TG,LDL-C,FPG,FINS,and HOMA-IR,while lower levels of HDL-C and HOMA-β compared with the control group;all differences were statistically significant(t-values ranged from 4.579 to 19.854,P<0.05).Correlation analysis showed a positive correlation between the levels of miR-423-5p and miR-328-3p(r=0.623,P<0.05).Both miR-423-5p and miR-328-3p were positively correlated with the insulin resistance-related indicators(TC,TG,LDL-C,FPG,FINS,HOMA-IR)(r-values ranged from 0.403 to 0.592,P<0.05),and negatively correlated with HDL-C and HOMA-β(r-values ranged from-0.523 to-0.368,P<0.05).Results of multivariate logistic regression analysis indicated that miR-423-5p,miR-328-3p,TC,TG,LDL-C,FPG,and FINS were all risk factors for GDM(OR values ranged from 2.120 to 4.851,P<0.05).Conclusion The levels of miR-423-5p and miR-328-3p are abnormally elevated in patients with GDM,and both are associated with insulin resistance.关键词
妊娠期糖尿病/miR-423-5p/miR-328-3p/胰岛素抵抗Key words
gestational diabetes mellitus/miR-423-5p/miR-328-3p/insulin resistance分类
医药卫生
