南方医科大学学报2026，Vol.46Issue(3)：513-522,10.DOI:10.12122/j.issn.1673-4254.2026.03.05
活血清解灵通过调控Sirt1-自噬信号通路减轻胆管结扎诱导的大鼠肝纤维化
Huoxue Qingjie Ling alleviates bile duct ligation-induced hepatic fibrosis in rats by regulating the Sirt1-autophagy signaling pathway
摘要
Abstract
Objective To investigate the protective effect of Huoxue Qingjie Ling(HXQJL)agaisnt bile duct ligation(BDL)-induced liver fibrosis in rats and the underlying mechanism.Methods SD rats were randomized into sham-operated group,BDL model group,low-and high-dose HXQJL treatment groups,Ex527(a Sirt1 inhibitor)group(EX527),and HXQJL+Ex527 group.Liver histopathological changes and collagen deposition were observed using HE and Sirius Red staining,and serum levels of ALT,AST,ALP,and GGT of the rats were measured using biochemical assays.The expression levels of α-SMA,FN,COL I,Atg5,Beclin1,p62,LC3B,and Sirt1 were determined by RT-PCR and Western blotting.Immunofluorescence staining was used to detect the expression of α-SMA,LC3B,and Sirt1.Results Compared with the sham-operated rats,the rats in BDL model group exhibited increased hepatocyte injury,inflammatory cell infiltration,and collagen deposition area with elevated serum levels of ALT,AST,ALP,and GGT,enhanced haptic expressions of α-SMA,FN,COL I,Atg5,and Beclin1,an increased LC3B-II/I ratio,and lowered hepatic expressions of p62 and Sirt1.All these changes were significantly alleviated in the two HXQJL treatment groups but aggravated in EX527 treatment group.Compared with those in high-dose HXQJL group,the protective effects of HXQJL on liver tissue injury,liver function,liver fibrosis,and autophagy were obviously mitigated by treatment with Ex527.Conclusion HXQJL has protective effect against BDL-induced liver fibrosis in rats by modulating the Sirt1-autophagy signaling pathway.关键词
活血清解灵/肝纤维化/自噬/Sirt1/EX527Key words
Huoxue Qingjie Ling/hepatic fibrosis/autophagy/Sirt1/EX527引用本文复制引用
李彩霞,崔立华,曹婕,樊宇星,周雪颖,张淑坤,左艳洁..活血清解灵通过调控Sirt1-自噬信号通路减轻胆管结扎诱导的大鼠肝纤维化[J].南方医科大学学报,2026,46(3):513-522,10.基金项目
天津市卫生健康委中医中西医结合重点项目(2023046) （2023046）
