海南医科大学学报2026，Vol.32Issue(5)：339-344,6.DOI:10.13210/j.cnki.jhmu.20250331.004
ANXA2通过抑制自死亡从而改善心肌细胞缺氧/复氧损伤的实验研究
ANXA2 ameliorates hypoxia/reoxygenation injury of cardiomyocytes by inhibiting autosis
摘要
Abstract
Objective:Autosis,as a form of autophagy dependent cell death,plays a contradictory role in promoting survival and promoting death in myocardial ischemia-reperfusion injury(MIRI).The aim of this study was to investigate the effect of an-nexin A2(ANXA2)on MIRI and its mechanism by means of autosis.Methods:Cell hypoxia/reoxygenation(H/R)injury model was used to simulate MIRI,shRNA(shANXA2)targeting intervention was constructed,cell activity was detected by CCK-8,the ratio of autophagosome to autophagolysosome was observed by confocal microscopy,and autosis related protein expression was detected by Western blot.Results:The expression of ANXA2 was significantly increased in cardiomyocytes after H/R inju-ry,the expression of transcription factor EB(TFEB)was increased after downregulating ANXA2 expression,the proportion of autophagosomes was decreased,the proportion of lysosomes was increased,and cell viability was restored.Western blot results showed that down-regulation of ANXA2 decreased the levels of ANXA2,P62 and LC3II,and up-regulated the expression of TFEB.Conclusions:ANXA2 inhibits the expression of TFEB and nuclear translocation,affects autophagy flux and the occur-rence of autosis,and aggravates the H/R injury of cardiomyocytes.Down-regulation of ANXA2 promotes autophagy flux and re-duces the occurrence of autosis,thereby alleviating MIRI.This study provides experimental evidence for ANXA2 as a potential target for the treatment of MIRI.关键词
自死亡/心肌缺血再灌注损伤/膜联蛋白A2(ANXA2)/转录因子EB(TFEB)/自噬通量Key words
Autosis/Myocardial ischemia-reperfusion injury/Annexin A2(ANXA2)/Transcription factor EB(TFEB)/Autophagic flux分类
医药卫生引用本文复制引用
许卫攀,李柳青,钟可文,金道群,刘滴..ANXA2通过抑制自死亡从而改善心肌细胞缺氧/复氧损伤的实验研究[J].海南医科大学学报,2026,32(5):339-344,6.基金项目
This study was supported by the Natural Science Foundation Project of Hubei Province(2022CFB159) （2022CFB159）
Hubei Province Health and Family Planning Scientific Research Project(WJ2023F061) （WJ2023F061）
Hubei Polytechnic University Research Project(22xjz6Q) （22xjz6Q）
Huangshi Central Hospital Research Project(ZX2023M02) 湖北省自然科学基金面上项目(2022CFB159) （ZX2023M02）
湖北省卫生健康委科研资助(WJ2023F061) （WJ2023F061）
湖北理工学院校级科研项目(22xjz6Q) （22xjz6Q）
黄石市中心医院科研项目(ZX2023M02) （ZX2023M02）
