海南医科大学学报2026，Vol.32Issue(5)：345-354,10.DOI:10.13210/j.cnki.jhmu.20251226.002
基于Keap1/Nrf2/HO-1信号轴探讨"治神调髓"针刺改善缺血性脑卒中大鼠氧化应激损伤的作用机制
Mechanism of"Zhishen Tiaosui"acupuncture on improving oxidative stress injury in rats with ischemic stroke based on the Keap1/Nrf2/HO-1 signaling axis
摘要
Abstract
Objective:To investigate the mechanism of"Zhishen Tiaosui"acupuncture improving oxidative stress injury in rats with ischemic stroke(IS)through the Keap1/Nrf2/HO-1 signaling axis.Methods:A total of 90 SPF male SD rats aged 6‒8 weeks(180‒220 g)were selected and divided into the sham operation group,the model group,the conventional acupuncture group,the"Zhishen Tiaosui"acupuncture group,and the Western medicine group(edaravone dexborneol group),the middle cerebral artery occlusion(MCAO)model by modified suture method were established,the neurological deficit score was evaluated by Zea-Longa neurological deficit score method,the cerebral infarction area was detected by TTC,the pathological damages of brain tissue were observed by H&E staining,the pathological changes of brain tissue were detected by Nissl staining,and the ultrastructure of isch-emic brain tissue neurons,including mitochondrial morphology,was observed by transmission electron microscopy.The contents of total superoxide dismutase(T-SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-PX),and reactive oxygen spe-cies(ROS)in serum were detected by ELISA.The protein expression levels of Keap1,Nrf2,and HO-1 in brain tissue were detect-ed by Western blot.Results:Compared to the model group,the neurological function of the rats in the"Zhishen Tiaosui"acupunc-ture group were significantly improved,and the infarct area was reduced.Neuronal damage was alleviated,the number of Nissl bodies was increased,and the ultrastructure was improved.The levels of T-SOD and GSH-PX in serum were increased(P<0.01),the contents of MDA and ROS were decreased(P<0.01),the expression level of Keap1 protein in brain tissue was de-creased(P<0.01),and the expression levels of Nrf2 and HO-1 proteins were increased(P<0.01).Conclusion:The"Zhishen Tiaosui"acupuncture may effectively reduce the oxidative stress injury of IS rats through the Keap1/Nrf2/HO-1 signaling axis.The therapeutic effect is comparable to that of edaravone dexborneol and is significantly better than that of conventional acupunc-ture.It provides a non-drug effective alternative for clinical practice.关键词
针刺/缺血性脑卒中/氧化应激/治神调髓Key words
Acupuncture/Ischemic stroke/Oxidative stress/Zhishen Tiaosui分类
医药卫生引用本文复制引用
李玲玲,徐银凤,谢姗珊,智勇..基于Keap1/Nrf2/HO-1信号轴探讨"治神调髓"针刺改善缺血性脑卒中大鼠氧化应激损伤的作用机制[J].海南医科大学学报,2026,32(5):345-354,10.基金项目
This study was supported by the National Natural Science Foundation of China(82560970,82060894) （82560970,82060894）
Xinjiang Uygur Autonomous Region Regional Collaborative Innovation Project(2022E01043) 国家自然科学基金(82560970,82060894) （2022E01043）
新疆维吾尔自治区区域协同创新专项(2022E01043) （2022E01043）
